ArticleNucleic acids research2016
Mapping DNA polymerase errors by single-molecule sequencing.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Extended sequence context shapes mutational bias inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- The A, B, C, D's of replicative DNA polymerase fidelity: utilizing high-throughput single-molecule sequencing to understand the molecular basis for DNA polymerase accuracy.Nucleic acids research · 2025Article
- Human DNA polymerase ε is a source of C>T mutations at CpG dinucleotides.Nature genetics · 2024Article
- Amplified DNA Heterogeneity Assessment with Oxford Nanopore Sequencing Applied to Cell Free Expression Templates.bioRxiv : the preprint server for biology · 2024Article
- Quantitative and systematic NMR measurements of sequence-dependent A-T Hoogsteen dynamics uncovers unique conformational specificity in the DNA double helix.bioRxiv : the preprint server for biology · 2024Article
- Amplified DNA heterogeneity assessment with Oxford Nanopore sequencing applied to cell free expression templates.PloS one · 2024Article
- Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCR.Microbiology spectrum · 2023Article
- Long-term whole blood DNA preservation by cost-efficient cryosilicification.Nature communications · 2022Article
- Temperature effect on polymerase fidelity.The Journal of biological chemistry · 2021Article
- Fidelity of a Bacterial DNA Polymerase in Microgravity, a Model for Human Health in Space.Frontiers in cell and developmental biology · 2021Article
- STAMP: a multiplex sequencing method for simultaneous evaluation of mitochondrial DNA heteroplasmies and content.NAR genomics and bioinformatics · 2020Article
- Metabarcoding a diverse arthropod mock community.Molecular ecology resources · 2019Article
- An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar.Genome biology · 2019Article
- Time-lapse imaging of molecular evolution by high-throughput sequencing.Nucleic acids research · 2018Article
- High-resolution mapping of DNA polymerase fidelity using nucleotide imbalances and next-generation sequencing.Nucleic acids research · 2018Article
- Indel-correcting DNA barcodes for high-throughput sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Detecting Rare Mutations and DNA Damage with Sequencing-Based Methods.Trends in biotechnology · 2018Review
- MERIT reveals the impact of genomic context on sequencing error rate in ultra-deep applications.BMC bioinformatics · 2018Article
- A Sequel to Sanger: amplicon sequencing that scales.BMC genomics · 2018Article
- An ultraprocessive, accurate reverse transcriptase encoded by a metazoan group II intron.RNA (New York, N.Y.) · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Genomic integrity is compromised by DNA polymerase replication errors, which occur in a sequence-dependent manner across the genome. Accurate and complete quantification of a DNA polymerase's error spectrum is challenging because errors are rare and difficult to detect. We report a high-throughput sequencing assay to map in vitro DNA replication errors at the single-molecule level. Unlike previous methods, our assay is able to rapidly detect a large number of polymerase errors at base resolution over any template substrate without quantification bias. To overcome the high error rate of high-throughput sequencing, our assay uses a barcoding strategy in which each replication product is tagged with a unique nucleotide sequence before amplification. This allows multiple sequencing reads of the same product to be compared so that sequencing errors can be found and removed. We demonstrate the ability of our assay to characterize the average error rate, error hotspots and lesion bypass fidelity of several DNA polymerases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.