ArticleNucleic acids research2016
Mutational analysis of the Potyviridae transcriptional slippage site utilized for expression of the P3N-PIPO and P1N-PISPO proteins.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Pooled it
- Preparation method shapes the recovery and ecological interpretation of DNA and RNA soil viral communities.Nature communications · 2026Article
- Nascent RNA profiling reveals genome-wide productive reiterative initiation regulating gene transcription in bacteria.Nucleic acids research · 2025Article
- Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection.Nature communications · 2025Article
- Article
- Mixed infection of ITPase-encoding potyvirid and secovirid in Mercurialis perennis: evidences for a convergent euphorbia-specific viral counterstrike.Virology journal · 2024Article
- Identification and characterization of theFrontiers in plant science · 2024Article
- Quantitative Analysis of RNA Polymerase Slippages for Production of P3N-PIPO Trans-frame Fusion Proteins in Potyvirids.Journal of microbiology (Seoul, Korea) · 2023Article
- Genome evolution of dengue virus serotype 1 under selection byVirus evolution · 2023Article
- Article
- P1 ofJournal of virology · 2021Article
- Article
- Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes.AIMS microbiology · 2020Review
- Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of theInternational journal of molecular sciences · 2019Article
- Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.The Journal of general virology · 2019Article
- Metagenomic Analysis of Plant Virus Occurrence in Common Bean (Frontiers in microbiology · 2018Article
- Cylindrical Inclusion Protein of Turnip Mosaic Virus Serves as a Docking Point for the Intercellular Movement of Viral Replication Vesicles.Plant physiology · 2017Article
- Dissecting the Molecular Mechanism of the Subcellular Localization and Cell-to-cell Movement of the Sugarcane mosaic virus P3N-PIPO.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Potyviridae comprise the largest and most important family of RNA plant viruses. An essential overlapping ORF, termed pipo, resides in an internal region of the main polyprotein ORF. Recently, expression of pipo was shown to depend on programmed transcriptional slippage at a conserved GAAAAAA sequence, resulting in the insertion of an extra A into a proportion of viral transcripts, fusing the pipo ORF in frame with the 5' third of the polyprotein ORF. However, the sequence features that mediate slippage have not been characterized. Using a duplicate copy of the pipo slip site region fused into a different genomic location where it can be freely mutated, we investigated the sequence requirements for transcriptional slippage. We find that the leading G is not strictly required, but increased flanking sequence GC content correlates with higher insertion rates. A homopolymeric hexamer is optimal for producing mainly single-nucleotide insertions. We also identify an overabundance of G to A substitutions immediately 3'-adjacent to GAAAAAA in insertion-free transcripts, which we infer to result from a 'to-fro' form of slippage during positive-strand synthesis. Analysis of wild-type and reverse complement sequences suggests that slippage occurs preferentially during synthesis of poly(A) and therefore occurs mainly during positive-strand synthesis.
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