ArticleNucleic acids research2016
Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Strand-seq and the future of personalized genomics.Nature genetics · 2026Review
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- Single-mitosis dissection of acute and chronic DNA mutagenesis and repair.Nature genetics · 2024Article
- Chromosome-Length Haplotypes with StrandPhaseR and Strand-seq.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling.Cell structure and function · 2021Article
- InvertypeR: Bayesian inversion genotyping with Strand-seq data.BMC genomics · 2021Article
- Construction of Whole Genomes from Scaffolds Using Single Cell Strand-Seq Data.International journal of molecular sciences · 2021Article
- Rate volatility and asymmetric segregation diversify mutation burden in cells with mutator alleles.Communications biology · 2021Article
- Single-cell analysis of structural variations and complex rearrangements with tri-channel processing.Nature biotechnology · 2020Article
- EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment.Frontiers in plant science · 2020Article
- Review
- Article
- The Response to DNA Damage at Telomeric Repeats and Its Consequences for Telomere Function.Genes · 2019Review
- Ring chromosomes: from formation to clinical potential.Protoplasma · 2018Review
- BLM helicase suppresses recombination at G-quadruplex motifs in transcribed genes.Nature communications · 2018Article
- Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq.eLife · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sister chromatid exchanges (SCEs) are considered sensitive indicators of genome instability. Detection of SCEs typically requires cells to incorporate bromodeoxyuridine (BrdU) during two rounds of DNA synthesis. Previous studies have suggested that SCEs are induced by DNA replication over BrdU-substituted DNA and that BrdU incorporation alone could be responsible for the high number of SCE events observed in cells from patients with Bloom syndrome (BS), a rare genetic disorder characterized by marked genome instability and high SCE frequency. Here we show using Strand-seq, a single cell DNA template strand sequencing technique, that the presence of variable BrdU concentrations in the cell culture medium and in DNA template strands has no effect on SCE frequency in either normal or BS cells. We conclude that BrdU does not induce SCEs and that SCEs detected in either normal or BS cells reflect DNA repair events that occur spontaneously.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.