Evidence map›Paper›PMID 27185878›Full record

ArticleMolecular and cellular biology2016

Novel MicroRNA Regulators of Atrial Natriuretic Peptide Production.

Connie Wu, Pankaj Arora, Obiajulu Agha, Liam A Hurst, Kaitlin Allen, Daniel I Nathan, Dongjian Hu, Pawina Jiramongkolchai, J Gustav Smith, Olle Melander and 8 more

Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03072602 (A Study of Racial Differences in Natriuretic Peptides Response to Glucose Challenge), which is not on this map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03072602 nacompletednot on this mapstarted 2017, after this paper: background citation

A Study of Racial Differences in Natriuretic Peptides Response to Glucose Challenge

TypeinterventionalSponsorUniversity of Alabama at BirminghamRan2017 to 2018Enrolled81ConditionsHealthy, Natriuretic Peptides, Metabolic SyndromeArmsStudy diet, Glucose Challenge
3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 8 institutions in 3 countries.

Connie WuCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Anesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Program in Medical and Population Genetics, Broad Institute, Cambridge, Massachusetts, USA.
Pankaj AroraCardiology Division, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Obiajulu AghaAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Liam A HurstAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Kaitlin AllenAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Daniel I NathanAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Dongjian HuCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
Pawina JiramongkolchaiAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
J Gustav SmithCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Program in Medical and Population Genetics, Broad Institute, Cambridge, Massachusetts, USA Center for Human Genetic Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Department of Cardiology, Clinical Sciences, Lund University, and Department of Heart Failure and Valvular Disease, Skåne University Hospital, Lund, Sweden.
Olle MelanderDepartment of Internal Medicine, Clinical Sciences, Lund University, and Skåne University Hospital, Malmö, Sweden.
Sander TrensonDepartment of Cardiovascular Sciences, University of Leuven, Leuven, Belgium.
Stefan P JanssensDepartment of Cardiovascular Sciences, University of Leuven, Leuven, Belgium.
Ibrahim DomianCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
Thomas J WangDivision of Cardiovascular Medicine, Department of Medicine, School of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
Kenneth D BlochCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Anesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Emmanuel S BuysAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA EBUYS@mgh.harvard.edu dbloch@partners.org.
Donald B BlochAnesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Division of Rheumatology, Allergy and Immunology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA EBUYS@mgh.harvard.edu dbloch@partners.org.
Christopher Newton-ChehCardiovascular Research Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA Program in Medical and Population Genetics, Broad Institute, Cambridge, Massachusetts, USA Center for Human Genetic Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Harvard University · USMassachusetts General Hospital · USKU Leuven · BEBroad Institute · USHarvard Stem Cell Institute · USLund University · SEUniversity of Alabama at Birmingham · USVanderbilt University · US

Funding

MULTIDISCIPLINARY RESEARCH TRAINING IN CARDIOLOGYT32HL007208 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI Patrick Thomas Ellinor, David E Sosnovik · 1985 to 2026
$16.1M
Physiologic effects of natriuretic peptide genetic variationR01HL098283 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI NEWTON-CHEH, CHRISTOPHER HOLMES, WANG, THOMAS J. · 2010 to 2013
$2.9M
Genetics of Cardiotoxic Drug Response to Psychiatric TherapyR01HL124262 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI NEWTON-CHEH, CHRISTOPHER HOLMES, PERLIS, ROY H. · 2015 to 2018
$2.7M
Physiologic Profiling of sGC Genetic VariantsR01HL113933 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI NEWTON-CHEH, CHRISTOPHER HOLMES · 2013 to 2016
$2.3M
Howard Hughes Medical InstituteNHLBI NIH HHS R01 HL098283NHLBI NIH HHS R01 HL113933NHLBI NIH HHS R01 HL124262NHLBI NIH HHS T32 HL007208
6 · The paper itself

Abstract

Atrial natriuretic peptide (ANP) has a central role in regulating blood pressure in humans. Recently, microRNA 425 (miR-425) was found to regulate ANP production by binding to the mRNA of NPPA, the gene encoding ANP. mRNAs typically contain multiple predicted microRNA (miRNA)-binding sites, and binding of different miRNAs may independently or coordinately regulate the expression of any given mRNA. We used a multifaceted screening strategy that integrates bioinformatics, next-generation sequencing data, human genetic association data, and cellular models to identify additional functional NPPA-targeting miRNAs. Two novel miRNAs, miR-155 and miR-105, were found to modulate ANP production in human cardiomyocytes and target genetic variants whose minor alleles are associated with higher human plasma ANP levels. Both miR-155 and miR-105 repressed NPPA mRNA in an allele-specific manner, with the minor allele of each respective variant conferring resistance to the miRNA either by disruption of miRNA base pairing or by creation of wobble base pairing. Moreover, miR-155 enhanced the repressive effects of miR-425 on ANP production in human cardiomyocytes. Our study combines computational, genomic, and cellular tools to identify novel miRNA regulators of ANP production that could be targeted to raise ANP levels, which may have applications for the treatment of hypertension or heart failure.

Indexed as

AllelesAtrial Natriuretic FactorBlood PressureCells, CulturedDown-RegulationFemaleGenetic VariationHumansMicroRNAsMyocytes, CardiacPlacentaPregnancyAtrial Natriuretic FactorMicroRNAsMIRN105 microRNA, humanMIRN155 microRNA, humanMIRN425 microRNA, humanNPPA protein, human

Identifiers

PMID27185878
PMCPMC4936060
OpenAlexW2396120510

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.