Evidence map›Paper›PMID 27185807›Full record

ArticleAntimicrobial agents and chemotherapy2016

In Vivo Rectal Mucosal Barrier Function Imaging in a Large-Animal Model by Using Confocal Endomicroscopy: Implications for Injury Assessment and Use in HIV Prevention Studies.

Gracie Vargas, Kathleen Listiak Vincent, Yong Zhu, David Szafron, Tyra Caitlin Brown, Paula Patricia Villarreal, Nigel Bourne, Gregg N Milligan, Massoud Motamedi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Quantitative imaging in medicine and surgery · 2023
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Gracie VargasDepartment of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, Texas, USA Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA grvargas@utmb.edu.
Kathleen Listiak VincentCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas, USA.
Yong ZhuCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA.
David SzafronCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA.
Tyra Caitlin BrownCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA.
Paula Patricia VillarrealCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA.
Nigel BourneDepartment of Pediatrics, University of Texas Medical Branch, Galveston, Texas, USA Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA.
Gregg N MilliganDepartment of Pediatrics, University of Texas Medical Branch, Galveston, Texas, USA Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA.
Massoud MotamediCenter for Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas, USA Department of Ophthalmology, University of Texas Medical Branch, Galveston, Texas, USA.
The University of Texas Medical Branch at Galveston · US

Funding

Development of novel quantitative methods for in vivo assessment of mucosal irritR01AI112015 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI MOTAMEDI, MASSOUD, VARGAS, GRACIE · 2014 to 2017
$2.4M
High-resolution optical imaging assessment of microbicide toxicityR33AI076062 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI MOTAMEDI, MASSOUD · 2009 to 2011
$1.7M
NIAID NIH HHS R01 AI112015NIAID NIH HHS R33 AI076062
6 · The paper itself

Abstract

Injury occurring on the surface of the rectal mucosal lining that causes defects in barrier function may result in increased risk for transmission of infection by HIV and other pathogens. Such injury could occur from microbicidal or other topical agents, mechanical trauma during consensual or nonconsensual intercourse, or inflammatory conditions. Tools for evaluation of rectal mucosal barrier function for assessing the mucosa under these conditions are lacking, particularly those that can provide in vivo structural and functional barrier integrity assessment and are adaptable to longitudinal imaging. We investigated confocal endomicroscopy (CE) as a means for in vivo imaging of the rectal epithelial barrier in the ovine model following spatially confined injury to the surface at a controlled site using a topical application of the microbicide test agent benzalkonium chloride. Topical and intravenous (i.v.) fluorescent probes were used with CE to provide subcellular resolution imaging of the mucosal surface and assessment of barrier function loss. A 3-point CE grading system based on cellular structure integrity and leakage of dye through the mucosa showed significant differences in score between untreated (1.19 ± 0.53) and treated (2.55 ± 0.75) tissue (P < 0.0001). Histological grading confirmed findings of barrier compromise. The results indicate that CE is an effective means for detecting epithelial injury and barrier loss following localized trauma in a large-animal model. CE is promising for real-time rectal mucosal evaluation after injury or trauma or topical application of emerging biomedical prevention strategies designed to combat HIV.

Indexed as

AnimalsDisease Models, AnimalHIV InfectionsIntestinal MucosaMicroscopy, ConfocalRectumSheep

Identifiers

PMID27185807
PMCPMC4958209
OpenAlexW2398351006

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.