ArticleNeuroscience2016
Immunostaining for Homer reveals the majority of excitatory synapses in laminae I-III of the mouse spinal dorsal horn.
Article in Neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Glutamatergic and GABAergic Synapses in the Human Spinal Dorsal Horn Revealed With Immunohistochemistry.The Journal of comparative neurology · 2025Article
- Article
- Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.Scientific reports · 2023Article
- γ-Protocadherins control synapse formation and peripheral branching of touch sensory neurons.Neuron · 2023Article
- Calretinin-expressing islet cells: a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.bioRxiv : the preprint server for biology · 2023Article
- Characterization of Fragile X Mental Retardation Protein expression in human nociceptors and their axonal projections to the spinal dorsal horn.The Journal of comparative neurology · 2023Article
- Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.Frontiers in molecular neuroscience · 2023Article
- Capsaicin-Induced Endocytosis of Endogenous Presynaptic CaFunction (Oxford, England) · 2023Article
- Pain hypersensitivity in a pharmacological mouse model of attention-deficit/hyperactivity disorder.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A novel spinal neuron connection for heat sensation.Neuron · 2022Article
- Neuroplasticity related to chronic pain and its modulation by microglia.Inflammation and regeneration · 2022Review
- Characterisation of deep dorsal horn projection neurons in the spinal cord of the Phox2a::Cre mouse line.Molecular pain · 2022Article
- The Anti-Inflammatory Agent Bindarit Attenuates the Impairment of Neural Development through Suppression of Microglial Activation in a Neonatal Hydrocephalus Mouse Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Studying IndependentThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Characterisation of lamina I anterolateral system neurons that express Cre in a Phox2a-Cre mouse line.Scientific reports · 2021Article
- Towards a Comprehensive Optical Connectome at Single Synapse ResolutionFrontiers in synaptic neuroscience · 2021Review
- Substance P-expressing Neurons in the Superficial Dorsal Horn of the Mouse Spinal Cord: Insights into Their Functions and their Roles in Synaptic Circuits.Neuroscience · 2020Article
- Expression of green fluorescent protein defines a specific population of lamina II excitatory interneurons in the GRP::eGFP mouse.Scientific reports · 2020Article
- Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The spinal dorsal horn processes somatosensory information before conveying it to the brain. The neuronal organization of the dorsal horn is still poorly understood, although recent studies have defined several distinct populations among the interneurons, which account for most of its constituent neurons. All primary afferents, and the great majority of neurons in laminae I-III are glutamatergic, and a major factor limiting our understanding of the synaptic circuitry has been the difficulty in identifying glutamatergic synapses with light microscopy. Although there are numerous potential targets for antibodies, these are difficult to visualize with immunocytochemistry, because of protein cross-linking following tissue fixation. Although this can be overcome by antigen retrieval methods, these lead to difficulty in detecting other antigens. The aim of this study was to test whether the postsynaptic protein Homer can be used to reveal glutamatergic synapses in the dorsal horn. Immunostaining for Homer gave punctate labeling when viewed by confocal microscopy, and this was restricted to synapses at the ultrastructural level. We found that Homer puncta were colocalized with the AMPA receptor GluR2 subunit, but not with the inhibitory synapse-associated protein gephyrin. We also examined several populations of glutamatergic axons and found that most boutons were in contact with at least one Homer punctum. These results suggest that Homer antibodies can be used to reveal the great majority of glutamatergic synapses without antigen retrieval. This will be of considerable value in tracing synaptic circuits, and also in investigating plasticity of glutamatergic synapses in pain states.
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