ArticleTransfusion2016
A targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti.
Article in Transfusion, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Structural and Functional Characterization of the 28 kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia Microti.Proteins · 2025Article
- A Highly Sensitive Multiplex Antibody Assay Reduces Window Period for Detection ofOpen forum infectious diseases · 2025Article
- Research progress on diagnostic techniques for differentFrontiers in cellular and infection microbiology · 2025Review
- Identification ofInfection and immunity · 2024Article
- The protective effects of BMSA1 and BMSA5-1-1 proteins against Babesia microti infection.Parasites, hosts and diseases · 2024Article
- Protective efficacy and correlates of immunity of immunodominant recombinantInfection and immunity · 2023Article
- Specific and Sensitive Diagnosis of Babesia microti Active Infection Using Monoclonal Antibodies to the Immunodominant Antigen BmGPI12.Journal of clinical microbiology · 2022Article
- Article
- Establishment and Application of an Indirect Enzyme-Linked Immunosorbent Assay for Measuring GPI-Anchored Protein 52 (P52) Antibodies inAnimals : an open access journal from MDPI · 2022Article
- Major Surface Antigens in ZoonoticPathogens (Basel, Switzerland) · 2022Review
- Identification and characterization of extracellular vesicles from red cells infected withFrontiers in cellular and infection microbiology · 2022Article
- Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogenFrontiers in cellular and infection microbiology · 2022Article
- Technologies for Detection ofPathogens (Basel, Switzerland) · 2021Review
- Review
- Antigen Discovery, Bioinformatics and Biological Characterization of Novel Immunodominant Babesia microti Antigens.Scientific reports · 2020Article
- Article
- Evidence for vesicle-mediated antigen export by the human pathogenLife science alliance · 2019Article
- A library of recombinant Babesia microti cell surface and secreted proteins for diagnostics discovery and reverse vaccinology.International journal for parasitology · 2019Article
- Analysis of the Babesia microti proteome in infected red blood cells by a combination of nanotechnology and mass spectrometry.International journal for parasitology · 2019Article
- Evaluation ofFrontiers in veterinary science · 2019Article
Corrections and comments
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Authors and funding
24 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundBabesia microti is a protozoan parasite responsible for the majority of reported cases of human babesiosis and a major risk to the blood supply. Laboratory screening of blood donors may help prevent transfusion-transmitted babesiosis but there is no Food and Drug Administration-approved screening method yet available. Development of a sensitive, specific, and highly automated B. microti antibody assay for diagnosis of acute babesiosis and blood screening could have an important impact on decreasing the health burden of B. microti infection. STUDY DESIGN AND
methodsHerein, we take advantage of recent advances in B. microti genomic analyses, field surveys of the reservoir host, and human studies in endemic areas to apply a targeted immunomic approach to the discovery of B. microti antigens that serve as signatures of active or past babesiosis infections. Of 19 glycosylphosphatidylinositol (GPI)-anchored protein candidates (BmGPI1-19) identified in the B. microti proteome, 17 were successfully expressed, printed on a microarray chip, and used to screen sera from uninfected and B. microti-infected mice and humans to determine immune responses that are associated with active and past infection.
resultsAntibody responses to various B. microti BmGPI antigens were detected and BmGPI12 was identified as the best biomarker of infection that provided high sensitivity and specificity when used in a microarray antibody assay.
conclusionBmGPI12 alone or in combination with other BmGPI proteins is a promising candidate biomarker for detection of B. microti antibodies that might be useful in blood screening to prevent transfusion-transmitted babesiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.