ArticleGenomics2016
HyCCAPP as a tool to characterize promoter DNA-protein interactions in Saccharomyces cerevisiae.
Article in Genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- A unified photosensitizer platform forbioRxiv : the preprint server for biology · 2026Article
- Selective identification of epigenetic regulators at methylated genomic sites by SelectID.Nature communications · 2025Article
- Review
- PROBER identifies proteins associated with programmable sequence-specific DNA in living cells.Nature methods · 2022Article
- Purification and enrichment of specific chromatin loci.Nature methods · 2020Review
- Decoding the chromatin proteome of a single genomic locus by DNA sequencing.PLoS biology · 2018Article
- Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells.Journal of visualized experiments : JoVE · 2018Article
- Elucidating the in vivo interactome of HIV-1 RNA by hybridization capture and mass spectrometry.Scientific reports · 2017Article
- Elucidating Protein-DNA Interactions in Human Alphoid Chromatin via Hybridization Capture and Mass Spectrometry.Journal of proteome research · 2017Article
- Multiplexed Sequence-Specific Capture of Chromatin and Mass Spectrometric Discovery of Associated Proteins.Analytical chemistry · 2017Article
Corrections and comments
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Authors and funding
25 authors at 3 institutions in 1 country.
Funding
Abstract
Currently available methods for interrogating DNA-protein interactions at individual genomic loci have significant limitations, and make it difficult to work with unmodified cells or examine single-copy regions without specific antibodies. In this study, we describe a physiological application of the Hybridization Capture of Chromatin-Associated Proteins for Proteomics (HyCCAPP) methodology we have developed. Both novel and known locus-specific DNA-protein interactions were identified at the ENO2 and GAL1 promoter regions of Saccharomyces cerevisiae, and revealed subgroups of proteins present in significantly different levels at the loci in cells grown on glucose versus galactose as the carbon source. Results were validated using chromatin immunoprecipitation. Overall, our analysis demonstrates that HyCCAPP is an effective and flexible technology that does not require specific antibodies nor prior knowledge of locally occurring DNA-protein interactions and can now be used to identify changes in protein interactions at target regions in the genome in response to physiological challenges.
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Registered trials
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