ArticlePflugers Archiv : European journal of physiology2016
Evidence for the functional involvement of members of the TRP channel family in the uptake of Na(+) and NH4 (+) by the ruminal epithelium.
Article in Pflugers Archiv : European journal of physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Sequence-based genome-wide association study reveals genetic and metabolic mechanisms underlying feed efficiency-related traits in beef cattle.Journal of animal science and biotechnology · 2026Article
- Menthol in Livestock: Unveiling Its Multifaceted Properties and Future Potential for Sustainable Agriculture.International journal of molecular sciences · 2025Review
- Prebiotics as modulators of colonic calcium and magnesium uptake.Acta physiologica (Oxford, England) · 2025Review
- The endocannabinoid system in bovine tissues: characterization of transcript abundance in the growing Holstein steer.BMC veterinary research · 2024Article
- Latest Insights into the In Vivo Studies in Murine Regarding the Role of TRP Channels in Wound Healing-A Review.International journal of molecular sciences · 2024Review
- A Network Pharmacology and Multi-Omics Combination Approach to Reveal the Effect of Strontium on CaInternational journal of molecular sciences · 2023Article
- TRPV3 Ion Channel: From Gene to Pharmacology.International journal of molecular sciences · 2023Review
- The effect of allyl isothiocyanate on chondrocyte phenotype is matrix stiffness-dependent: Possible involvement of TRPA1 activation.Frontiers in molecular biosciences · 2023Article
- Commentary on: Liebe, F., Liebe, H., Sponder, G., Mergler, S., Stumpff, F. "Effects of butyrate on ruminal CaPflugers Archiv : European journal of physiology · 2022Article
- Effects of butyratePflugers Archiv : European journal of physiology · 2022Article
- Thymol Disrupts Cell Homeostasis and Inhibits the Growth ofContrast media & molecular imaging · 2022Article
- Beyond CaPflugers Archiv : European journal of physiology · 2021Article
- The TRPA1 Agonist Cinnamaldehyde Induces the Secretion of HCOInternational journal of molecular sciences · 2021Article
- Butyrate Permeation across the Isolated Ovine Reticulum Epithelium.Animals : an open access journal from MDPI · 2020Article
- The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport.Pflugers Archiv : European journal of physiology · 2020Article
- Dietary supplementation of menthol-rich bioactive lipid compounds alters circadian eating behaviour of sheep.BMC veterinary research · 2019Article
- Unravelling the secrets of the caecum.Pflugers Archiv : European journal of physiology · 2019Article
- Dietary supplementation of essential oils in dairy cows: evidence for stimulatory effects on nutrient absorption.Animal : an international journal of animal bioscience · 2019Article
- Effects of dietary menthol-rich bioactive lipid compounds on zootechnical traits, blood variables and gastrointestinal function in growing sheep.Journal of animal science and biotechnology · 2019Article
- Ureases in the gastrointestinal tracts of ruminant and monogastric animals and their implication in urea-N/ammonia metabolism: A review.Journal of advanced research · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large quantities of protein are degraded in the fermentative parts of the gut to ammonia, which is absorbed, detoxified to urea, and excreted, leading to formation of nitrogenous compounds such as N2O that are associated with global warming. In ruminants, channel-mediated uptake of NH4 (+) from the rumen predominates. The molecular identity of these channels remains to be clarified. Ruminal cells and epithelia from cows and sheep were investigated using patch clamp, Ussing chamber, microelectrode techniques, and qPCR. In patch clamp experiments, bovine ruminal epithelial cells expressed a conductance for NH4 (+) that could be blocked in a voltage-dependent manner by divalent cations. In the native epithelium, NH4 (+) depolarized the apical potential, acidified the cytosol and induced a rise in short-circuit current (I sc) that persisted after the removal of Na(+), was blocked by verapamil, enhanced by the removal of divalent cations, and was sensitive to certain transient receptor potential (TRP) channel modulators. Menthol or thymol stimulated the I sc in Na(+) or NH4 (+) containing solutions in a dose-dependent manner and modulated transepithelial Ca(2+) fluxes. On the level of messenger RNA (mRNA), ovine and bovine ruminal epithelium expressed TRPA1, TRPV3, TRPV4, TRPM6, and TRPM7, with any expression of TRPV6 marginal. No bands were detected for TRPV1, TRPV5, or TRPM8. Functional and molecular biological data suggest that the transport of NH4 (+), Na(+), and Ca(2+) across the rumen involves TRP channels, with TRPV3 and TRPA1 emerging as prime candidate genes. TRP channels may also contribute to the transport of NH4 (+) across other epithelia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.