ArticleScientific reports2016
Nuclease Footprints in Sperm Project Past and Future Chromatin Regulatory Events.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- A father's legacy: the sperm epigenome, preimplantation development, and paternal environment.Epigenomics · 2025Review
- Paternal Effects in Mammalian Reproduction: Functional, Environmental, and Clinical Relevance of Sperm Components in Early Embryos and Beyond.Molecular reproduction and development · 2025Review
- Article
- Gene expression levels modulate germline mutation rates through the compound effects of transcription-coupled repair and damage.Human genetics · 2022Article
- Higher Germline Mutagenesis of Genes with Stronger Testis Expressions Refutes the Transcriptional Scanning Hypothesis.Molecular biology and evolution · 2020Article
- The Interplay Between Replacement and Retention of Histones in the Sperm Genome.Frontiers in genetics · 2020Review
- Transgenerational epigenetic reprogramming of early embryos: a mechanistic model.Environmental epigenetics · 2020Review
- Article
- Human Sperm Chromosomes: To Form Hairpin-Loops, Or Not to Form Hairpin-Loops, That Is the Question.Genes · 2019Article
- RNA element discovery from germ cell to blastocyst.Nucleic acids research · 2019Article
- Localization of dimethylated histone three lysine four in the Rattus norvegicus sperm genome.Biology of reproduction · 2018Article
- Chips off the Old Block: How a Father's Preconception Exposures Might Affect the Health of His Children.Environmental health perspectives · 2018Article
- Identification of a variant-specific phosphorylation of TH2A during spermiogenesis.Scientific reports · 2017Article
- Testis-specific transcriptional regulators selectively occupy BORIS-bound CTCF target regions in mouse male germ cells.Scientific reports · 2017Article
- Epigenetic reprogramming of the zygote in mice and men: on your marks, get set, go!Reproduction (Cambridge, England) · 2016Review
- The Epigenetic Consequences of Paternal Exposure to Environmental Contaminants and Reproductive Toxicants.Current environmental health reports · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear remodeling to a condensed state is a hallmark of spermatogenesis. This is achieved by replacement of histones with protamines. Regions retaining nucleosomes may be of functional significance. To determine their potential roles, sperm from wild type and transgenic mice harboring a single copy insert of the human protamine cluster were subjected to Micrococcal Nuclease-seq. CENTIPEDE, a hierarchical Bayesian model, was used to identify multiple spatial patterns, "footprints", of MNase-seq reads along the sperm genome. Regions predicted by CENTIPEDE analysis to be bound by a regulatory factor in sperm were correlated with genomic landmarks and higher order chromatin structure datasets to identify potential roles for these factors in regulating either prior or post spermatogenic, i.e., early embryonic events. This approach linked robust endogenous protamine transcription and transgene suppression to its chromatin environment within topologically associated domains. Of the candidate enhancer-bound regulatory proteins, Ctcf, was associated with chromatin domain boundaries in testes and embryonic stem cells. The continuity of Ctcf binding through the murine germline may permit rapid reconstitution of chromatin organization following fertilization. This likely reflects its preparation for early zygotic genome activation and comparatively accelerated preimplantation embryonic development program observed in mouse as compared to human and bull.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.