ArticleScientific reports2016
Vitamin-D receptor agonist calcitriol reduces calcification in vitro through selective upregulation of SLC20A2 but not SLC20A1 or XPR1.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- A Novel Frameshift Mutation inBiomedicines · 2026Article
- Astrocytes in Primary Familial Brain Calcification (PFBC): Emphasis on the Importance of Induced Pluripotent Stem Cell-Derived Human Astrocyte Models.Advances in experimental medicine and biology · 2025Review
- Metabolites and metabolism in vascular calcification: links between adenosine signaling and the methionine cycle.American journal of physiology. Heart and circulatory physiology · 2024Review
- First-time seizure revealing late-onset Fahr's disease: a case report and brief literature review.Frontiers in human neuroscience · 2024Article
- The clinical and genetic spectrum of primary familial brain calcification.Journal of neurology · 2023Article
- The Pathology of Primary Familial Brain Calcification: Implications for Treatment.Neuroscience bulletin · 2023Review
- Insights into pulmonary phosphate homeostasis and osteoclastogenesis emerge from the study of pulmonary alveolar microlithiasis.Nature communications · 2023Article
- Expression of phosphate and calcium transporters and their regulators in parotid glands of mice.Pflugers Archiv : European journal of physiology · 2023Article
- Decoding brain calcifications: A single-center descriptive case series and examination of pathophysiological mechanisms.SAGE open medical case reports · 2023Article
- Intracranial Calcification and Seizure with Down Syndrome: A Case Report.JNMA; journal of the Nepal Medical Association · 2022Article
- MiR-9-5p Regulates Genes Linked to Cerebral Calcification in the Osteogenic Differentiation Model and Induces Generalized Alteration in the Ion Channels.Journal of molecular neuroscience : MN · 2021Article
- Developmental Changes in Phosphate Homeostasis.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Article
- Isolation and Characterization of Three Sodium-Phosphate Cotransporter Genes and Their Transcriptional Regulation in the Grass CarpInternational journal of molecular sciences · 2020Article
- Mechanisms of calcification in Fahr disease and exposure of potential therapeutic targets.Neurology. Clinical practice · 2020Review
- A likely pathogenic variant in theAnnals of clinical and translational neurology · 2019Article
- PiT-2, a type III sodium-dependent phosphate transporter, protects against vascular calcification in mice with chronic kidney disease fed a high-phosphate diet.Kidney international · 2018Article
- Calcitriol Reverses the Down-Regulation Pattern of Tuberous Sclerosis Complex Genes in an In Vitro Calcification Model.Journal of molecular neuroscience : MN · 2018Article
- Phosphate Transporters Expression in Patients with Primary Familial Brain Calcifications.Journal of molecular neuroscience : MN · 2017Article
- Inherited Arterial Calcification Syndromes: Etiologies and Treatment Concepts.Current osteoporosis reports · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin D deficiency (hypovitaminosis D) causes osteomalacia and poor long bone mineralization. In apparent contrast, hypovitaminosis D has been reported in patients with primary brain calcifications ("Fahr's disease"). We evaluated the expression of two phosphate transporters which we have found to be associated with primary brain calcification (SLC20A2, whose promoter has a predicted vitamin D receptor binding site, and XPR1), and one unassociated (SLC20A1), in an in vitro model of calcification. Expression of all three genes was significantly decreased in calcifying human bone osteosarcoma (SaOs-2) cells. Further, we confirmed that vitamin D (calcitriol) reduced calcification as measured by Alizarin Red staining. Cells incubated with calcitriol under calcifying conditions specifically maintained expression of the phosphate transporter SLC20A2 at higher levels relative to controls, by RT-qPCR. Neither SLC20A1 nor XPR1 were affected by calcitriol treatment and remained suppressed. Critically, knockdown of SLC20A2 gene and protein with CRISPR technology in SaOs2 cells significantly ablated vitamin D mediated inhibition of calcification. This study elucidates the mechanistic importance of SLC20A2 in suppressing the calcification process. It also suggests that vitamin D might be used to regulate SLC20A2 gene expression, as well as reduce brain calcification which occurs in Fahr's disease and normal aging.
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