ArticleScientific reports2016
Spatial organization of heterologous metabolic system in vivo based on TALE.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review.Microbial cell factories · 2018Pooled it
- Engineering conditional protein-protein interactions for dynamic cellular control.Biotechnology advances · 2024Review
- Application of artificial scaffold systems in microbial metabolic engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Dynamic modulation of enzyme activity by synthetic CRISPR-Cas6 endonucleases.Nature chemical biology · 2022Article
- Synthetic Scaffold Systems for Increasing the Efficiency of Metabolic Pathways in Microorganisms.Biology · 2021Review
- Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective.Frontiers in bioengineering and biotechnology · 2021Review
- Protein Engineering for Improving and Diversifying Natural Product Biosynthesis.Trends in biotechnology · 2020Review
- Enzyme Assembly for Compartmentalized Metabolic Flux Control.Metabolites · 2020Review
- Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale.Frontiers in bioengineering and biotechnology · 2020Review
- Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes.Plant physiology · 2019Review
- Optimizing the localization of astaxanthin enzymes for improved productivity.Biotechnology for biofuels · 2018Article
- Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation.Biotechnology advancesReview
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For years, prokaryotic hosts have been widely applied in bio-engineering. However, the confined in vivo enzyme clustering of heterologous metabolic pathways in these organisms often results in low local concentrations of enzymes and substrates, leading to a low productive efficacy. We developed a new method to accelerate a heterologous metabolic system by integrating a transcription activator-like effector (TALE)-based scaffold system into an Escherichia coli chassis. The binding abilities of the TALEs to the artificial DNA scaffold were measured through ChIP-PCR. The effect of the system was determined through a split GFP study and validated through the heterologous production of indole-3-acetic acid (IAA) by incorporating TALE-fused IAA biosynthetic enzymes in E. coli. To the best of our knowledge, we are the first to use the TALE system as a scaffold for the spatial organization of bacterial metabolism. This technique might be used to establish multi-enzymatic reaction programs in a prokaryotic chassis for various applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.