ArticleBMC genomics2016
Small RNA sequencing and degradome analysis of developing fibers of short fiber mutants Ligon-lintles-1 (Li 1 ) and -2 (Li 2 ) revealed a role for miRNAs and their targets in cotton fiber elongation.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding.Frontiers in plant science · 2024Review
- A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.Plant physiology · 2022Article
- Integrated transcriptome and small RNA sequencing in revealing miRNA-mediated regulatory network of floral bud break inFrontiers in plant science · 2022Article
- A Modified Actin (Gly65Val Substitution) Expressed in Cotton Disrupts Polymerization of Actin Filaments Leading to the Phenotype of Ligon Lintless-1 (International journal of molecular sciences · 2021Article
- Investigation of thePlants (Basel, Switzerland) · 2020Article
- Analysis of the MIR160 gene family and the role of MIR160a_A05 in regulating fiber length in cotton.Planta · 2019Article
- Long non-coding RNAs and their potential functions in Ligon-lintless-1 mutant cotton during fiber development.BMC genomics · 2019Article
- Fine mapping and identification of the fuzzless gene GaFzl in DPL972 (Gossypium arboreum).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Identification and profiling of upland cotton microRNAs at fiber initiation stage under exogenous IAA application.BMC genomics · 2019Article
- MicroRNA profiling analysis of developing berries for 'Kyoho' and its early-ripening mutant during berry ripening.BMC plant biology · 2018Article
- Deciphering microRNAs and Their Associated Hairpin Precursors in a Non-Model Plant, Abelmoschus esculentus.Non-coding RNA · 2017Article
- Genome-wide identification and characterization of microRNAs differentially expressed in fibers in a cotton phytochrome A1 RNAi line.PloS one · 2017Article
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Authors and funding
6 authors.
Funding
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Abstract
backgroundThe length of cotton fiber is an important agronomic trait that directly affects the quality of yarn and fabric. Understanding the molecular basis of fiber elongation would provide a means for improvement of fiber length. Ligon-lintless-1 (Li 1 ) and -2 (Li 2 ) are monogenic and dominant mutations that result in an extreme reduction in the length of lint fiber on mature seeds. In a near-isogenic state with wild type cotton these two short fiber mutants provide an effective model system to study the mechanisms of fiber elongation. Plant miRNAs regulate many aspects of growth and development. However, the mechanism underlying the miRNA-mediated regulation of fiber development is largely unknown.
resultsSmall RNA libraries constructed from developing fiber cells of the short fiber mutants Li 1 and Li 2 and their near-isogenic wild type lines were sequenced. We identified 24 conservative and 147 novel miRNA families with targets that were detected through degradome sequencing. The distribution of the target genes into functional categories revealed the largest set of genes were transcription factors. Expression profiles of 20 miRNAs were examined across a fiber developmental time course in wild type and short fiber mutations. We conducted correlation analysis between miRNA transcript abundance and the length of fiber for 11 diverse Upland cotton lines. The expression patterns of 4 miRNAs revealed significant negative correlation with fiber lengths of 11 cotton lines.
conclusionsOur results suggested that the mutations have changed the regulation of miRNAs expression during fiber development. Further investigations of differentially expressed miRNAs in the Li 1 and Li 2 mutants will contribute to better understanding of the regulatory mechanisms of cotton fiber development. Four miRNAs negatively correlated with fiber length are good candidates for further investigations of miRNA regulation of important genotype dependent fiber traits. Thus, our results will contribute to further studies on the role of miRNAs in cotton fiber development and will provide a tool for fiber improvement through molecular breeding.
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