ArticleNature structural & molecular biology2016
Mechanism of extracellular ion exchange and binding-site occlusion in a sodium/calcium exchanger.
Article in Nature structural & molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.
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- Molecular mechanisms of mitochondrial CaNature communications · 2026Article
- Molecular mechanisms of mitochondrial CabioRxiv : the preprint server for biology · 2026Article
- ActivityFinder: Toward the Fully Automatic Integration of Structural and Binding Affinity Data.Journal of chemical information and modeling · 2026Article
- Uterine fluid proteomic and metabolomic profiles associated with soft-shelled egg formation in laying hens.Frontiers in veterinary science · 2026Article
- Structural and functional mapping of ion access pathways in the human KChannels (Austin, Tex.) · 2025Article
- Structural basis of CAX1 autoinhibition by its amino-terminal domain in Arabidopsis thaliana.Nature plants · 2025Article
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- Structural mechanisms of PIPeLife · 2025Article
- Dataset from a human-in-the-loop approach to identify functionally important protein residues from literature.Scientific data · 2024Article
- Developing and Benchmarking Sulfate and Sulfamate Force Field Parameters via Ab Initio Molecular Dynamics Simulations To Accurately Model Glycosaminoglycan Electrostatic Interactions.Journal of chemical information and modeling · 2024Article
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- Conformational free-energy landscapes of a NaProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural dynamics of NaCommunications biology · 2024Article
- SLC24A-mediated calcium exchange as an indispensable component of the diatom cell density-driven signaling pathway.The ISME journal · 2024Article
- Structural insight into the allosteric inhibition of human sodium-calcium exchanger NCX1 by XIP and SEA0400.The EMBO journal · 2024Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Na(+)/Ca(2+) exchangers use the Na(+) electrochemical gradient across the plasma membrane to extrude intracellular Ca(2+) and play a central role in Ca(2+) homeostasis. Here, we elucidate their mechanisms of extracellular ion recognition and exchange through a structural analysis of the exchanger from Methanococcus jannaschii (NCX_Mj) bound to Na(+), Ca(2+) or Sr(2+) in various occupancies and in an apo state. This analysis defines the binding mode and relative affinity of these ions, establishes the structural basis for the anticipated 3:1 Na(+)/Ca(2+)-exchange stoichiometry and reveals the conformational changes at the onset of the alternating-access transport mechanism. An independent analysis of the dynamics and conformational free-energy landscape of NCX_Mj in different ion-occupancy states, based on enhanced-sampling molecular dynamics simulations, demonstrates that the crystal structures reflect mechanistically relevant, interconverting conformations. These calculations also reveal the mechanism by which the outward-to-inward transition is controlled by the ion occupancy, thereby explaining the emergence of strictly coupled Na(+)/Ca(2+) antiport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.