ArticlePloS one2016
Cyclic AMP Receptor Protein Acts as a Transcription Regulator in Response to Stresses in Deinococcus radiodurans.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 30 citations in OpenAlex.
- Rising from the fragments: a multi-factorial regulatory framework of DNA damage response in Deinococcus radiodurans.Extremophiles : life under extreme conditions · 2026Review
- Specific role of two NlpC/P60 endopeptidases in cell division and membrane vesicle formation inCurrent research in microbial sciences · 2026Article
- cAMP-independent Crp homolog adds to the multi-layer regulatory network inFrontiers in cellular and infection microbiology · 2025Article
- Radiotolerant endophytic bacteria and analysis of the effects ofBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
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- Comparative genomics ofFrontiers in microbiology · 2024Article
- Unleashing the Influence of cAMP Receptor Protein: The Master Switch of Bacteriocin Export inInternational journal of molecular sciences · 2023Article
- A putative bifunctional CPD/ (6-4) photolyase from the cyanobacteriaFrontiers in microbiology · 2022Article
- Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic BacteriumMicroorganisms · 2020Article
- Construction, analysis and validation of co-expression network to understand stress adaptation in Deinococcus radiodurans R1.PloS one · 2020Article
- PprI: The Key Protein in Response to DNA Damage inFrontiers in cell and developmental biology · 2020Review
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- Malate-Dependent Carbon Utilization Enhances Central Metabolism and Contributes to Biological Fitness ofFrontiers in microbiology · 2019Article
- Conservation and diversity of radiation and oxidative stress resistance mechanisms in Deinococcus species.FEMS microbiology reviews · 2019Review
- DdrI, a cAMP Receptor Protein Family Member, Acts as a Major Regulator for Adaptation of Deinococcus radiodurans to Various Stresses.Journal of bacteriology · 2018Article
- A Genome-Wide Search for Ionizing-Radiation-Responsive Elements in Deinococcus radiodurans Reveals a Regulatory Role for the DNA Gyrase Subunit A Gene's 5' Untranslated Region in the Radiation and Desiccation Response.Applied and environmental microbiology · 2017Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cyclic AMP receptor protein family of transcription factors regulates various metabolic pathways in bacteria, and also play roles in response to environmental changes. Here, we identify four homologs of the CRP family in Deinococcus radiodurans, one of which tolerates extremely high levels of oxidative stress and DNA-damaging reagents. Transcriptional levels of CRP were increased under hydrogen peroxide (H2O2) treatment during the stationary growth phase, indicating that CRPs function in response to oxidative stress. By constructing all CRP single knockout mutants, we found that the dr0997 mutant showed the lowest tolerance toward H2O2, ultraviolet radiation, ionizing radiation, and mitomycin C, while the phenotypes of the dr2362, dr0834, and dr1646 mutants showed slight or no significant differences from those of the wild-type strain. Taking advantage of the conservation of the CRP-binding site in many bacteria, we found that transcription of 18 genes, including genes encoding chromosome-partitioning protein (dr0998), Lon proteases (dr0349 and dr1974), NADH-quinone oxidoreductase (dr1506), thiosulfate sulfurtransferase (dr2531), the DNA repair protein UvsE (dr1819), PprA (dra0346), and RecN (dr1447), are directly regulated by DR0997. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that certain genes involved in anti-oxidative responses, DNA repair, and various cellular pathways are transcriptionally attenuated in the dr0997 mutant. Interestingly, DR0997 also regulate the transcriptional levels of all CRP genes in this bacterium. These data suggest that DR0997 contributes to the extreme stress resistance of D. radiodurans via its regulatory role in multiple cellular pathways, such as anti-oxidation and DNA repair pathways.
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