ArticlePloS one2016
Transcriptome Sequencing of Chemically Induced Aquilaria sinensis to Identify Genes Related to Agarwood Formation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Integrated proteomic and metabolomic analyses reveal the salt-induced agarwood formation mechanism of Aquilaria sinensis.BMC plant biology · 2025Article
- Biosynthesis and Its Regulatory Mechanisms of 2-(2-Phenylethyl)chromones in Agarwood.Plants (Basel, Switzerland) · 2025Review
- De novo transcriptome assembly and analysis during agarwood induction in Gyrinops versteegii Gilg. seedling.Scientific reports · 2025Article
- Towards sustainable agarwood production: integrating microbial interactions, anatomical changes, and metabolite biosynthesis.Journal of industrial microbiology & biotechnology · 2024Review
- Article
- Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome.Frontiers in plant science · 2024Article
- Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process inInternational journal of molecular sciences · 2023Article
- Article
- Integrated Transcriptomic, Metabolomic, and Physiological Analyses Reveal New Insights into Fragrance Formation in the Heartwood ofInternational journal of molecular sciences · 2022Article
- Comparative transcriptome analysis reveals different defence responses during the early stage of wounding stress in Chi-Nan germplasm and ordinary Aquilaria sinensis.BMC plant biology · 2022Article
- Rediscovering the Therapeutic Potential of Agarwood in the Management of Chronic Inflammatory Diseases.Molecules (Basel, Switzerland) · 2022Review
- Effects of various artificial agarwood-induction techniques on the metabolome of Aquilaria sinensis.BMC plant biology · 2021Article
- Transcriptome of Aquilaria malaccensis containing agarwood formed naturally and induced artificially.BMC research notes · 2021Article
- Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused byInternational journal of molecular sciences · 2019Article
- Time-based LC-MS/MS analysis provides insights into early responses to mechanical wounding, a major trigger to agarwood formation inRSC advances · 2019Article
- Chemical Profiles of Cultivated Agarwood Induced by Different Techniques.Molecules (Basel, Switzerland) · 2019Article
- Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera.Scientific reports · 2019Article
- Agarwood Induction: Current Developments and Future Perspectives.Frontiers in plant science · 2019Review
- The Scent of Stress: Evidence From the Unique Fragrance of Agarwood.Frontiers in plant science · 2019Review
- De Novo Transcriptome Sequencing of the Deep-Sea-Derived FungusInternational journal of molecular sciences · 2018Article
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Authors and funding
10 authors.
Funding
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Abstract
backgroundAgarwood is a traditional Chinese medicine used as a clinical sedative, carminative, and antiemetic drug. Agarwood is formed in Aquilaria sinensis when A. sinensis trees are threatened by external physical, chemical injury or endophytic fungal irritation. However, the mechanism of agarwood formation via chemical induction remains unclear. In this study, we characterized the transcriptome of different parts of a chemically induced A. sinensis trunk sample with agarwood. The Illumina sequencing platform was used to identify the genes involved in agarwood formation. METHODOLOGY/PRINCIPAL
findingsA five-year-old Aquilaria sinensis treated by formic acid was selected. The white wood part (B1 sample), the transition part between agarwood and white wood (W2 sample), the agarwood part (J3 sample), and the rotten wood part (F5 sample) were collected for transcriptome sequencing. Accordingly, 54,685,634 clean reads, which were assembled into 83,467 unigenes, were obtained with a Q20 value of 97.5%. A total of 50,565 unigenes were annotated using the Nr, Nt, SWISS-PROT, KEGG, COG, and GO databases. In particular, 171,331,352 unigenes were annotated by various pathways, including the sesquiterpenoid (ko00909) and plant-pathogen interaction (ko03040) pathways. These pathways were related to sesquiterpenoid biosynthesis and defensive responses to chemical stimulation. CONCLUSIONS/SIGNIFICANCE: The transcriptome data of the different parts of the chemically induced A. sinensis trunk provide a rich source of materials for discovering and identifying the genes involved in sesquiterpenoid production and in defensive responses to chemical stimulation. This study is the first to use de novo sequencing and transcriptome assembly for different parts of chemically induced A. sinensis. Results demonstrate that the sesquiterpenoid biosynthesis pathway and WRKY transcription factor play important roles in agarwood formation via chemical induction. The comparative analysis of the transcriptome data of agarwood and A. sinensis lays the foundation for elucidating the mechanism of agarwood formation via chemical induction, and thus, enables future improvements in agarwood quality while protecting endangered wild A. sinensis.
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