Evidence map›Paper›PMID 27182429›Full record

ArticleAdvances in optics and photonics2015

A pragmatic guide to multiphoton microscope design.

Michael D Young, Jeffrey J Field, Kraig E Sheetz, Randy A Bartels, Jeff Squier

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advances in optics and photonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. TWINKLE: An open-source two-photon microscope for teaching and research.bioRxiv : the preprint server for biology · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michael D YoungCenter for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA.
Jeffrey J FieldW. M. Keck Laboratory for Raman Imaging of Cell-to-Cell Communications, Colorado State University, Fort Collins, Colorado 80523, USA; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Kraig E SheetzPhotonics Research Center, Department of Physics and Nuclear Engineering, United States Military Academy, West Point, New York 10996, USA.
Randy A BartelsW. M. Keck Laboratory for Raman Imaging of Cell-to-Cell Communications, Colorado State University, Fort Collins, Colorado 80523, USA; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Jeff SquierCenter for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA.

Funding

Manipulating Neural Tissue With Ultra-Short Laser PulsesR01EB003832 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KAUFHOLD, JOHN, KLEINFELD, DAVID · 2005 to 2014
$8.2M
NIBIB NIH HHS R01 EB003832
6 · The paper itself

Abstract

Multiphoton microscopy has emerged as a ubiquitous tool for studying microscopic structure and function across a broad range of disciplines. As such, the intent of this paper is to present a comprehensive resource for the construction and performance evaluation of a multiphoton microscope that will be understandable to the broad range of scientific fields that presently exploit, or wish to begin exploiting, this powerful technology. With this in mind, we have developed a guide to aid in the design of a multiphoton microscope. We discuss source selection, optical management of dispersion, image-relay systems with scan optics, objective-lens selection, single-element light-collection theory, photon-counting detection, image rendering, and finally, an illustrated guide for building an example microscope.

Identifiers

PMID27182429
PMCPMC4863715

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.