Evidence map›Paper›PMID 27182286›Full record

ArticleConservation genetics resources2016

Rapid Identification of Major Histocompatibility Complex Class I Haplotypes Using Deep Sequencing in an Endangered Old World Monkey.

Noah D Simons, Maria Jose Ruiz-Lopez, Colin A Chapman, Tony L Goldberg, Julie A Karl, Roger W Wiseman, Patrick S Bohn, David H O'Connor, Nelson Ting

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Conservation genetics resources, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Noah D SimonsDepartment of Anthropology, University of Oregon, Eugene, OR 97405, USA.
Maria Jose Ruiz-LopezInstitute of Ecology and Evolution, University of Oregon, Eugene, OR 97405, USA.
Colin A ChapmanDepartment of Anthropology and McGill School of Environment, Montreal, Quebec, H3A 2T7, Canada.
Tony L GoldbergDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53725, USA; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
Julie A KarlWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
Roger W WisemanWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
Patrick S BohnWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
David H O'ConnorDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53715, USA; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
Nelson TingDepartment of Anthropology, University of Oregon, Eugene, OR 97405, USA; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97405, USA.
University of Wisconsin–Madison · USUniversity of Oregon · USMcGill University · CA

Funding

Biological and Human Dimensions of Primate Retroviral TransmissionR01AI098420 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI GOLDBERG, TONY L. · 2011 to 2015
$2.3M
NIAID NIH HHS R01 AI098420
6 · The paper itself

Abstract

Immunogenetic data from wild primate populations have been difficult to obtain, due to logistic and methodological constraints. We applied a well-characterized deep sequencing method for MHC I typing, developed for macaques, to a population of wild red colobus to assess the feasibility of identifying MHC I-A/B haplotypes. Ten individuals produced sufficient data from blood and tissue samples to assign haplotypes. Eighty-two sequences were classified as red colobus MHC I alleles distributed across six MHC I loci. Individuals averaged ~13k reads across six MHC I loci, with 83% of all alleles representing either MHC I-A or MHC I-B loci. This study not only represents an important advance in the identification and genotyping of MHC in the endangered red colobus but also shows the potential for using this approach in other endangered wild primates.

Indexed as

immunogeneticsMajor Histocompatibility Complexnext generation sequencingprimatesred colobus

Identifiers

PMID27182286
PMCPMC4863455
OpenAlexW2257812733

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.