Evidence map›Paper›PMID 27181261›Full record

ArticleJournal of diabetes investigation2016

Transplantation of dental pulp stem cells suppressed inflammation in sciatic nerves by promoting macrophage polarization towards anti-inflammation phenotypes and ameliorated diabetic polyneuropathy.

Maiko Omi, Masaki Hata, Nobuhisa Nakamura, Megumi Miyabe, Yasuko Kobayashi, Hideki Kamiya, Jiro Nakamura, Shogo Ozawa, Yoshinobu Tanaka, Jun Takebe and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes investigation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 1 synthesis or guideline pooled it, 87 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. WNT4 reprograms dental pulp stem cells to resist PANoptosis and rebuild neurogenic potential for facial nerve injury repair.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
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  8. Diabetic neuropathy: cutting-edge research and future directions.Signal transduction and targeted therapy · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Maiko OmiDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Masaki HataDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Nobuhisa NakamuraDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Megumi MiyabeDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Yasuko KobayashiDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Hideki KamiyaDivision of Diabetes, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute, Japan.
Jiro NakamuraDivision of Diabetes, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute, Japan.
Shogo OzawaDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Yoshinobu TanakaDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Jun TakebeDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Tatsuaki MatsubaraDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Keiko NaruseDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Aichi Gakuin University · JPAichi Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

AIMS/

introductionDental pulp stem cells (DPSCs) are thought to be an attractive candidate for cell therapy. We recently reported that the transplantation of DPSCs increased nerve conduction velocity and nerve blood flow in diabetic rats. In the present study, we investigated the immunomodulatory effects of DPSC transplantation on diabetic peripheral nerves. MATERIALS AND

methodsDPSCs were isolated from the dental pulp of Sprague-Dawley rats and expanded in culture. Eight weeks after the streptozotocin injection, DPSCs were transplanted into the unilateral hindlimb skeletal muscles. Four weeks after DPSC transplantation, neurophysiological measurements, inflammatory gene expressions and the number of CD68-positive cells in sciatic nerves were assessed. To confirm the immunomodulatory effects of DPSCs, the effects of DPSC-conditioned media on lipopolysaccharide-stimulated murine macrophage RAW264.7 cells were investigated.

resultsDiabetic rats showed significant delays in sciatic nerve conduction velocities and decreased sciatic nerve blood flow, all of which were ameliorated by DPSC transplantation. The number of CD68-positive monocytes/macrophages and the gene expressions of M1 macrophage-expressed cytokines, tumor necrosis factor-α and interleukin-1β, were increased in the sciatic nerves of the diabetic rats. DPSC transplantation significantly decreased monocytes/macrophages and tumor necrosis factor-α messenger ribonucleic acid expression, and increased the gene expression of the M2 macrophage marker, CD206, in the sciatic nerves of the diabetic rats. The in vitro study showed that DPSC-conditioned media significantly increased the gene expressions of interleukin-10 and CD206 in lipopolysaccharide-stimulated RAW264.7 cells.

conclusionsThese results suggest that DPSC transplantation promoted macrophages polarization towards anti-inflammatory M2 phenotypes, which might be one of the therapeutic mechanisms for diabetic polyneuropathy.

Indexed as

AnimalsCell PolarityCell ProliferationCell SurvivalDental PulpDiabetes Mellitus, ExperimentalDiabetic NeuropathiesDisease Models, AnimalMacrophagesMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationNeural ConductionNeuritisPhenotypeRatsCell transplantationDental pulp stem cellsDiabetic polyneuropathy

Identifiers

PMID27181261
PMCPMC4931198
OpenAlexW2232558079

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.