Evidence map›Paper›PMID 27180907›Full record

ArticleCell2016

A Mechanogenetic Toolkit for Interrogating Cell Signaling in Space and Time.

Daeha Seo, Kaden M Southard, Ji-Wook Kim, Hyun Jung Lee, Justin Farlow, Jung-Uk Lee, David B Litt, Thomas Haas, A Paul Alivisatos, Jinwoo Cheon and 2 more

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
12.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 171 citations in OpenAlex.

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  11. Cellular and Molecular Effects of Magnetic Fields.International journal of molecular sciences · 2024
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  20. Multi-targeted immunotherapeutics to treat B cell malignancies.Journal of controlled release : official journal of the Controlled Release Society · 2023
    Review

23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Daeha SeoDepartment of Otolaryngology, University of California, San Francisco, San Francisco, CA 94115, USA; Department of Chemistry and Department of Materials Sciences and Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Kavli Energy NanoScience Institute, University of California, Berkeley and Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Kaden M SouthardDepartment of Otolaryngology, University of California, San Francisco, San Francisco, CA 94115, USA; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
Ji-Wook KimCenter for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea; Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
Hyun Jung LeeDepartment of Otolaryngology, University of California, San Francisco, San Francisco, CA 94115, USA.
Justin FarlowDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
Jung-Uk LeeCenter for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea; Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
David B LittDepartment of Chemistry and Department of Materials Sciences and Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Kavli Energy NanoScience Institute, University of California, Berkeley and Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Thomas HaasDepartment of Otolaryngology, University of California, San Francisco, San Francisco, CA 94115, USA.
A Paul AlivisatosDepartment of Chemistry and Department of Materials Sciences and Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Kavli Energy NanoScience Institute, University of California, Berkeley and Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Jinwoo CheonCenter for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea; Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
Zev J GartnerDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: zev.gartner@ucsf.edu.
Young-Wook JunDepartment of Otolaryngology, University of California, San Francisco, San Francisco, CA 94115, USA; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea; Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: young-wook.jun@ucsf.edu.
University of California, San Francisco · USInstitute for Basic Science · KRKavli Energy NanoScience Institute · USLawrence Berkeley National Laboratory · USYonsei University · KR

Funding

Rewiring Therapeutic Cells: User-Controlled T Cells and Stem CellsP50GM081879 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIM, WENDELL A · 2010 to 2017
$19.0M
RESEARCH TRAINING IN CHEMISTRY AND CHEMICAL BIOLOGYT32GM064337 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CRAIK, CHARLES SCOTT · 2002 to 2021
$7.8M
Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Total synthesis of the human mammary glandDP2HD080351 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN · 2013 to 2013
$2.4M
Spatiotemporal Control of Dynamic Notch Signaling with Subcellular ResolutionR01GM112081 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JUN, YOUNG-WOOK · 2014 to 2018
$1.5M
Single Molecule Imaging of Guided Axonal Development using Plasmon NanorulersR21EB015088 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JUN, YOUNG-WOOK · 2012 to 2013
$414k
Small, modular, and monovalent Quantum Dots for single molecule imaging of NotchR21EB018044 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN, JUN, YOUNG-WOOK · 2014 to 2015
$411k
NIBIB NIH HHS R21 EB015088NIBIB NIH HHS R21 EB018044NICHD NIH HHS DP2 HD080351NIGMS NIH HHS P50 GM081879NIGMS NIH HHS R01 GM112081NIGMS NIH HHS T32 GM064337NIGMS NIH HHS T32 GM066698
6 · The paper itself

Abstract

Tools capable of imaging and perturbing mechanical signaling pathways with fine spatiotemporal resolution have been elusive, despite their importance in diverse cellular processes. The challenge in developing a mechanogenetic toolkit (i.e., selective and quantitative activation of genetically encoded mechanoreceptors) stems from the fact that many mechanically activated processes are localized in space and time yet additionally require mechanical loading to become activated. To address this challenge, we synthesized magnetoplasmonic nanoparticles that can image, localize, and mechanically load targeted proteins with high spatiotemporal resolution. We demonstrate their utility by investigating the cell-surface activation of two mechanoreceptors: Notch and E-cadherin. By measuring cellular responses to a spectrum of spatial, chemical, temporal, and mechanical inputs at the single-molecule and single-cell levels, we reveal how spatial segregation and mechanical force cooperate to direct receptor activation dynamics. This generalizable technique can be used to control and understand diverse mechanosensitive processes in cell signaling. VIDEO ABSTRACT.

Indexed as

Genetic TechniquesMechanotransduction, CellularMetal NanoparticlesActinsCadherinsCell LineCells, CulturedHumansMechanoreceptorsMicrospheresMolecular Probe TechniquesReceptors, NotchRecombinant Fusion ProteinsSpatial AnalysisTimeActinsCadherinsReceptors, NotchRecombinant Fusion Proteins

Identifiers

PMID27180907
PMCPMC4892966
OpenAlexW2355190364

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.