ArticleJournal of virological methods2016
Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of different animal species.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Molecular epidemiology of group A rotavirus (RVA) and bovine coronavirus (BCoV) associated with neonatal calf diarrhea in Türkiye.Tropical animal health and production · 2026Article
- Whole-genome characterization and zoonotic insights of feline rotavirus A genotypes G3P[9] and novel G6P[9] circulating in domestic cats in Thailand.Veterinary world · 2026Article
- A pilot study on a heterologous prime-boost approach for inducing an immune response against double-layered human rotavirus particles.BMC research notes · 2025Article
- Advances in diagnosis of diseases causing diarrhea in newborn calves.Veterinary research communications · 2025Review
- RT-PCR-based assessment of the SD Bioline Rota/Adeno Antigen-based test in infants with and without diarrhea.Virology journal · 2023Article
- Article
- Article
- Rapid Detection ofInternational journal of molecular sciences · 2022Article
- High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms.Frontiers in veterinary science · 2022Article
- Determination of an RT-qPCR viral load cutoff point for the etiologic diagnosis of rotavirus A diarrhea in neonate dairy calves.Frontiers in veterinary science · 2022Article
- Detection and evaluation of rotavirus surveillance methods as viral indicator in the aquatic environments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Benign Convulsions with Mild Rotavirus and Norovirus Gastroenteritis: Nationwide Data from the Health Insurance Review and Assessment Service in South Korea.Children (Basel, Switzerland) · 2021Article
- Rotavirus in Calves and Its Zoonotic Importance.Veterinary medicine international · 2021Review
- RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms.PloS one · 2021Article
- Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens.Pathogens (Basel, Switzerland) · 2020Article
- Molecular surveillance of human rotaviruses in drinking water and investigation of the efficiency of their removal in Isfahan water treatment plant.Environmental monitoring and assessment · 2019Article
- Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan.BMC gastroenterology · 2019Article
- Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus.BMC veterinary research · 2019Article
- Evaluation of Targeted Next-Generation Sequencing for Detection of Bovine Pathogens in Clinical Samples.Journal of clinical microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is no gold standard for detection of Rotavirus Group A (RVA), one of the main causes of diarrhea in neonatal animals. Sensitive and specific real-time RT-PCR (rtRT-PCR) assays are available for RVA but require submission of the clinical samples to diagnostic laboratories. Patient-side immunoassays for RVA protein detection have shown variable results, particularly with samples from unintended species. A sensitive and specific test for detection of RVA on the farm would facilitate rapid management decisions. The insulated isothermal RT-PCR (RT-iiPCR) assay works in a portable machine to allow sensitive and specific on-site testing. The aim of this investigation was to evaluate a commercially available RT-iiPCR assay for RVA detection in feces from different animal species. This assay was compared to an in-house rtRT-PCR assay and a commercially available rtRT-PCR kit, as well as an ELISA and EM for RVA detection. All three PCR assays targeted the well-conserved NSP5 gene. Clinical fecal samples from 108 diarrheic animals (mainly cattle and horses) were tested. The percentage of positive samples by ELISA, EM, in-house rtRT-PCR, commercial rtRT-PCR, and RT-iiPCR was 29.4%, 31%, 36.7%, 51.4%, 56.9%, respectively. The agreement between different assays was high (81.3-100%) in samples containing high viral loads. The sensitivity of the RT-iiPCR assay appeared to be higher than the commercially available rtRT-PCR assay, with a limit of detection (95% confidence index) of 3-4 copies of in vitro transcribed dsRNA. In conclusion, the user-friendly, field-deployable RT-iiPCR system holds substantial promise for on-site detection of RVA.
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