ArticleInsect biochemistry and molecular biology2016
Distribution of cuticular proteins in different structures of adult Anopheles gambiae.
Article in Insect biochemistry and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
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- Assessment of MALDI-TOF MS for Arthropod Identification Based on Exuviae Spectra Analysis.Biological procedures online · 2025Article
- Cataract induction in an arthropod reveals how lens crystallins contribute to the formation of biological glass.PloS one · 2025Article
- Specialized structure and function of the apical extracellular matrix at sense organs.Cells & development · 2024Review
- Genetics of flight in spongy moths (Lymantria dispar ssp.): functionally integrated profiling of a complex invasive trait.BMC genomics · 2024Article
- Knockdown ofFrontiers in cellular and infection microbiology · 2024Article
- Mosquitoes Possess Specialized Cuticular Proteins That Are Evolutionarily Related to the Elastic Protein Resilin.Insects · 2023Article
- Global Gene Expression Analysis Reveals Complex Cuticle Organization of the Tribolium Compound Eye.Genome biology and evolution · 2023Article
- The Blimp-1 transcription factor acts in non-neuronal cells to regulate terminal differentiation of the Drosophila eye.Development (Cambridge, England) · 2022Article
- Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis ofInternational journal of molecular sciences · 2022Article
- Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs.BMC genomics · 2021Article
- Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph inInsects · 2021Article
- Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.Communications biology · 2021Article
- Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator.Insect science · 2020Article
- Cuticular Structure Proteomics in the Pea AphidJournal of proteome research · 2020Article
- Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization.iScience · 2020Article
- Insect Cuticular Chitin Contributes to Form and Function.Current pharmaceutical design · 2020Article
- Proteomics reveals localization of cuticular proteins in Anopheles gambiae.Insect biochemistry and molecular biology · 2019Article
- Profiles of soluble proteins in chemosensory organs of three members of the afro-tropical Anopheles gambiae complex.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2017Article
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6 authors.
Funding
Abstract
Anopheles gambiae devotes over 2% (295) of its protein coding genes to structural cuticular proteins (CPs) that have been classified into 13 different families plus ten low complexity proteins not assigned to families. Small groups of genes code for identical proteins reducing the total number of unique cuticular proteins to 282. Is the large number because different structures utilize different CPs, or are all of the genes widely expressed? We used LC-MS/MS to learn how many products of these genes were found in five adult structures: Johnston's organs, the remainder of the male antennae, eye lenses, legs, and wings. Data were analyzed against both the entire proteome and a smaller database of just CPs. We recovered unique peptides for 97 CPs and shared peptides for another 35. Members of 11 of the 13 families were recovered as well as some unclassified. Only 11 CPs were present exclusively in only one structure while 43 CPs were recovered from all five structures. A quantitative analysis, using normalized spectral counts, revealed that only a few CPs were abundant in each structure. When the MS/MS data were run against the entire proteome, the majority of the top hits were to CPs, but peptides were recovered from an additional 467 proteins. CP peptides were frequently recovered from chitin-binding domains, confirming that protein-chitin interactions are not mediated by covalent bonds. Comparison with three other MS/MS analyses of cuticles or cuticle-rich structures augmented the current analysis. Our findings provide new insights into the composition of different mosquito structures and reveal the complexity of selection and utilization of genes coding for structural cuticular proteins.
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