Evidence map›Paper›PMID 27179561›Full record

ArticleBiosensors & bioelectronics2016

Selective and sensitive detection of MiRNA-21 based on gold-nanorod functionalized polydiacetylene microtube waveguide.

Yu Zhu, Dong Qiu, Guang Yang, Mengqiao Wang, Qijin Zhang, Pei Wang, Hai Ming, Douguo Zhang, Yue Yu, Gang Zou and 2 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics.International journal of molecular sciences · 2023
    Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yu ZhuCAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
Dong QiuDepartment of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
Guang YangCAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
Mengqiao WangCAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
Qijin ZhangCAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
Pei WangDepartment of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
Hai MingDepartment of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
Douguo ZhangDepartment of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China. Electronic address: dgzhang@ustc.edu.cn.
Yue YuDivision of Gastroenterology, Affiliated Provincial Hospital, Anhui Medical University, No.17 Lu Jiang Road, Hefei, Anhui 230001, PR China. Electronic address: yuyuemd@yahoo.com.cn.
Gang ZouCAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China. Electronic address: gangzou@ustc.edu.cn.
Ramachandram BaduguCenter for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.
Joseph R LakowiczCenter for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.

Funding

Plasmon-Controlled Fluorescence and Cardiac MarkersR01EB006521 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2007 to 2015
$2.7M
Diffusion-Enhanced Lanthanide Nanoparticle FRET AssaysR21GM107986 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2014 to 2016
$568k
Bioaffinity Assays Using UV One-Dimensional Photonic Crystals (1DPC)R21EB018959 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2015 to 2016
$420k
Multi-User Time-Resolved Fluorescence SpectrometerS10OD019975 · OD · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2015 to 2015
$243k
NIBIB NIH HHS R01 EB006521NIBIB NIH HHS R21 EB018959NIGMS NIH HHS R21 GM107986NIH HHS S10 OD019975
6 · The paper itself

Abstract

Development of rapid, highly selective and sensitive miRNA detection in a complex biological environment has attracted considerable attention. Herein, we describe a novel two step method to construct gold-nanorod functionalized polydiacetylene (PDA) microtube for miRNA detection. In PDA microtube, with a one-dimensional (1D) waveguide nature, the excitation position and emission out-coupling position are far apart, thus helpful in reducing contribution of auto-fluorescence from biological sample. The use of specially designed toehold-mediated strand displacement reaction enables the reliable and selective discrimination of miRNA sequences with high sequence homology. Based on the condensing enrichment effect, the detection limit of the proposed PDA microtube system is as low as 0.01nM, and it can be applied directly to detect disease-specific miRNA targets in human serum. This PDA microtube waveguide system can be further integrated into the chip for the potential applications in minimally invasive, portable clinical diagnostic equipment.

Indexed as

Biosensing TechniquesFluorescenceGoldHumansLimit of DetectionMicroRNAsNanotubesPolyacetylene PolymerPolymersPolyynesGoldMicroRNAsMIRN21 microRNA, humanPolyacetylene PolymerpolydiacetylenePolymersPolyynesBiosensingMicroRNAOptical waveguidePolydiacetylene

Identifiers

PMID27179561
PMCPMC4983514

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.