Evidence map›Paper›PMID 27179433›Full record

ArticleBiomaterials2016

In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging.

Kvar C L Black, Aida Ibricevic, Sean P Gunsten, Jeniree A Flores, Tiffany P Gustafson, Jeffery E Raymond, Sandani Samarajeewa, Ritu Shrestha, Simcha E Felder, Tianyi Cai and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Radionuclide-Activated Nanomaterials and Their Biomedical Applications.Angewandte Chemie (International ed. in English) · 2019
    Review
  8. Multimodality Imaging Agents with PET as the Fundamental Pillar.Angewandte Chemie (International ed. in English) · 2019
    Review
  9. Article
  10. Article
  11. Molecular Imaging in Nanotechnology and Theranostics.Molecular imaging and biology · 2017
    Review
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Kvar C L BlackDepartment of Radiology, Washington University, St. Louis, MO 63110, USA.
Aida IbricevicDepartment of Internal Medicine, Washington University, St. Louis, MO 63110, USA.
Sean P GunstenDepartment of Internal Medicine, Washington University, St. Louis, MO 63110, USA.
Jeniree A FloresDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA.
Tiffany P GustafsonDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA.
Jeffery E RaymondDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA; Laboratory for Synthetic-Biologic Interactions, Texas A&M University, College Station, TX 77843, USA.
Sandani SamarajeewaDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Ritu ShresthaDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Simcha E FelderDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Tianyi CaiDepartment of Internal Medicine, Washington University, St. Louis, MO 63110, USA.
Yuefei ShenDepartment of Chemistry, Washington University, St. Louis, MO 63110, USA.
Ann-Kathrin LöbsDepartment of Internal Medicine, Washington University, St. Louis, MO 63110, USA.
Natalia ZhegalovaDepartment of Radiology, Washington University, St. Louis, MO 63110, USA.
Deborah H SultanDepartment of Radiology, Washington University, St. Louis, MO 63110, USA.
Mikhail BerezinDepartment of Radiology, Washington University, St. Louis, MO 63110, USA.
Karen L WooleyDepartment of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA; Laboratory for Synthetic-Biologic Interactions, Texas A&M University, College Station, TX 77843, USA.
Yongjian LiuDepartment of Radiology, Washington University, St. Louis, MO 63110, USA.
Steven L BrodyDepartment of Radiology, Washington University, St. Louis, MO 63110, USA; Department of Internal Medicine, Washington University, St. Louis, MO 63110, USA. Electronic address: brodys@wustl.edu.

Funding

NHLBI NIH HHS HHSN268201000046C
6 · The paper itself

Abstract

Nanoparticles (NPs) play expanding roles in biomedical applications including imaging and therapy, however, their long-term fate and clearance profiles have yet to be fully characterized in vivo. NP delivery via the airway is particularly challenging, as the clearance may be inefficient and lung immune responses complex. Thus, specific material design is required for cargo delivery and quantitative, noninvasive methods are needed to characterize NP pharmacokinetics. Here, biocompatible poly(acrylamidoethylamine)-b-poly(dl-lactide) block copolymer-based degradable, cationic, shell-cross-linked knedel-like NPs (Dg-cSCKs) were employed to transfect plasmid DNA. Radioactive and optical beacons were attached to monitor biodistribution and imaging. The preferential release of cargo in acidic conditions provided enhanced transfection efficiency compared to non-degradable counterparts. In vivo gene transfer to the lung was correlated with NP pharmacokinetics by radiolabeling Dg-cSCKs and performing quantitative biodistribution with parallel positron emission tomography and Čerenkov imaging. Quantitation of imaging over 14 days corresponded with the pharmacokinetics of NP movement from the lung to gastrointestinal and renal routes, consistent with predicted degradation and excretion. This ability to noninvasively and accurately track NP fate highlights the advantage of incorporating multifunctionality into particle design.

Indexed as

LuminescencePositron-Emission TomographyAnimalsDNAHEK293 CellsHumansHydrogen-Ion ConcentrationIodine RadioisotopesLungMiceMultimodal ImagingNanoparticlesPlasmidsSolutionsTissue DistributionTransfectionDNAIodine RadioisotopesSolutionsBiodistributionČerenkov luminescence imagingCytotoxicityDegradable nanoparticleGene expressionLung

Identifiers

PMID27179433
PMCPMC4899101

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.