ArticleNucleic acids research2016
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Light-dependent processes in algae across scales: from cellular physiology to ecological organization.Plant physiology · 2026Review
- Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025Review
- A rapid aureochrome opto-switch enables diatom acclimation to dynamic light.Nature communications · 2024Article
- A Knockout of the Photoreceptor PtAureo1a Results in Altered Diel Expression of Diatom Clock Components.Plants (Basel, Switzerland) · 2024Article
- More than a photoreceptor: aureochromes are intrinsic to the diatom light-regulated transcriptional network.Journal of experimental botany · 2024Article
- Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum.Journal of experimental botany · 2024Article
- Genome-Wide Identification and Analysis of the Aureochrome Gene Family inPlants (Basel, Switzerland) · 2022Article
- Dimerization processes for light-regulated transcription factor Photozipper visualized by high-speed atomic force microscopy.Scientific reports · 2022Article
- Aureochromes maintain polyunsaturated fatty acid content in Nannochloropsis oceanica.Plant physiology · 2022Article
- Transmission of light signals from the light-oxygen-voltage core via the hydrophobic region of the β-sheet surface in aureochrome-1.Scientific reports · 2021Article
- Diel transcriptional oscillations of light-sensitive regulatory elements in open-ocean eukaryotic plankton communities.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms.iScience · 2020Article
- In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells.The Journal of biological chemistry · 2020Article
- Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.The Plant cell · 2020Review
- PhaeoNet: A Holistic RNAseq-Based Portrait of Transcriptional Coordination in the Model DiatomFrontiers in plant science · 2020Article
- bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatomProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Noncanonical Chromophore Reveals Structural Rearrangements of the Light-Oxygen-Voltage Domain upon Photoactivation.Biochemistry · 2019Article
- Arguments for an additional long-lived intermediate in the photocycle of the full-length aureochrome 1c receptor: A time-resolved small-angle X-ray scattering study.Structural dynamics (Melville, N.Y.) · 2019Article
- Variation in LOV Photoreceptor Activation Dynamics Probed by Time-Resolved Infrared Spectroscopy.Biochemistry · 2018Article
- Quantitative analyses of the equilibria among DNA complexes of a blue-light-regulated bZIP module, Photozipper.Biophysics and physicobiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modular architecture of aureochrome blue light receptors, found in several algal groups including diatoms, is unique by having the LOV-type photoreceptor domain fused to the C-terminus of its putative effector, an N-terminal DNA-binding bZIP module. The structural and functional understanding of aureochromes' light-dependent signaling mechanism is limited, despite their promise as an optogenetic tool. We show that class I aureochromes 1a and 1c from the diatom Phaeodactylum tricornutum are regulated in a light-independent circadian rhythm. These aureochromes are capable to form functional homo- and heterodimers, which recognize the ACGT core sequence within the canonical 'aureo box', TGACGT, in a light-independent manner. The bZIP domain holds a more folded and less flexible but extended conformation in the duplex DNA-bound state. FT-IR spectroscopy in the absence and the presence of DNA shows light-dependent helix unfolding in the LOV domain, which leads to conformational changes in the bZIP region. The solution structure of DNA bound to aureochrome points to a tilted orientation that was further validated by molecular dynamics simulations. We propose that aureochrome signaling relies on an allosteric pathway from LOV to bZIP that results in conformational changes near the bZIP-DNA interface without major effects on the binding affinity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.