Evidence map›Paper›PMID 27178573›Full record

ArticleMolecular biology reports2016

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek's disease lymphoma cell proliferation and migration.

Bo Han, Ling Lian, Xin Li, Chunfang Zhao, Lujiang Qu, Changjun Liu, Jiuzhou Song, Ning Yang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Bo HanDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Ling LianDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Xin LiDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Chunfang ZhaoDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Lujiang QuDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Changjun LiuDivision of Avian Infectious Diseases, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
Jiuzhou SongDepartment of Animal & Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
Ning YangDepartment of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China. nyang@cau.edu.cn.
China Agricultural University · CNHarbin Veterinary Research Institute · CNUniversity of Maryland, College Park · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marek's disease (MD) is an infectious disease of chickens caused by MD virus (MDV), which is a herpesvirus that initiates tumor formation. Studies have indicated that microRNAs (miRNAs) are linked with the development of cancers or tumors. Previously, gga-miR-130a was discovered downregulated in MDV-infected tissues. Here, we aimed to explore the further function of gga-miR-130a in MD. The expression of gga-miR-130a in MDV-infected and uninfected spleens was detected by quantitative real-time PCR (qRT-PCR). Subsequently, proliferation and migration assays of MDV-transformed lymphoid cells (MSB1) were carried out by transfecting gga-miR-130a. The target genes of gga-miR-130a were predicted using TargetScan and miRDB and clustered through Gene Ontology analysis. The target genes were validated by western blot, qRT-PCR, and a dual luciferase reporter assay. Our results show that the expression of gga-miR-130a was reduced in MDV-infected spleens. Gga-miR-130a showed an inhibitory effect on MSB1 cell proliferation and migration. Two target genes, homeobox A3 (HOXA3) and MyoD family inhibitor domain containing (MDFIC), were predicted and clustered to cell proliferation. Results indicate that gga-miR-130a regulates HOXA3 and MDFIC at the protein level but not at the mRNA level. Moreover, the gga-miR-130a binding sites of two target genes have been confirmed. We conclude that gga-miR-130a can arrest MSB1 cell proliferation and migration, and target HOXA3 and MDFIC, which are both involved in the regulation of cell proliferation. Collectively, gga-miR-130a plays a critical role in the tumorigenesis associated with chicken MD.

Indexed as

3' Untranslated RegionsAnimalsAvian ProteinsBinding SitesCell Line, TumorCell MovementCell ProliferationChickensGene ExpressionHerpesvirus 2, GallidHomeodomain ProteinsMarek DiseaseMicroRNAsMyogenic Regulatory FactorsRNA InterferenceSpleen3' Untranslated RegionsAvian ProteinsHomeodomain ProteinsMicroRNAsMyogenic Regulatory FactorsCell migrationCell proliferationChickengga-miR-130aHOXA3Marek’s diseaseMDFIC

Identifiers

PMID27178573
OpenAlexW2355266299

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.