Evidence map›Paper›PMID 27178552›Full record

ReviewAnalytical and bioanalytical chemistry2016

Advances in DNA metabarcoding for food and wildlife forensic species identification.

Martijn Staats, Alfred J Arulandhu, Barbara Gravendeel, Arne Holst-Jensen, Ingrid Scholtens, Tamara Peelen, Theo W Prins, Esther Kok

Abstract readReview
In one paragraph

Review in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed.

  1. Article
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  9. Review
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  11. Investigating the Microbial Dynamics ofFoods (Basel, Switzerland) · 2025
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  15. Article
  16. Review
  17. Article
  18. Article
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  20. Review

25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Martijn StaatsRIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands. martijn.staats@wur.nl.
Alfred J ArulandhuRIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.
Barbara GravendeelNaturalis Biodiversity Center, Sylviusweg 72, P.O. Box 9517, Leiden, The Netherlands.
Arne Holst-JensenNorwegian Veterinary Institute, Ullevaalsveien 68, P.O. Box 750, Sentrum, 0106, Oslo, Norway.
Ingrid ScholtensRIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.
Tamara PeelenDutch Customs Laboratory, Kingsfordweg 1, 1043 GN, Amsterdam, The Netherlands.
Theo W PrinsRIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.
Esther KokRIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Species identification using DNA barcodes has been widely adopted by forensic scientists as an effective molecular tool for tracking adulterations in food and for analysing samples from alleged wildlife crime incidents. DNA barcoding is an approach that involves sequencing of short DNA sequences from standardized regions and comparison to a reference database as a molecular diagnostic tool in species identification. In recent years, remarkable progress has been made towards developing DNA metabarcoding strategies, which involves next-generation sequencing of DNA barcodes for the simultaneous detection of multiple species in complex samples. Metabarcoding strategies can be used in processed materials containing highly degraded DNA e.g. for the identification of endangered and hazardous species in traditional medicine. This review aims to provide insight into advances of plant and animal DNA barcoding and highlights current practices and recent developments for DNA metabarcoding of food and wildlife forensic samples from a practical point of view. Special emphasis is placed on new developments for identifying species listed in the Convention on International Trade of Endangered Species (CITES) appendices for which reliable methods for species identification may signal and/or prevent illegal trade. Current technological developments and challenges of DNA metabarcoding for forensic scientists will be assessed in the light of stakeholders' needs.

Indexed as

FoodForensic GeneticsAnimalsAnimals, WildComputational BiologyDNADNA Barcoding, TaxonomicHigh-Throughput Nucleotide SequencingPlantsDNAConvention on International Trade of Endangered SpeciesCytochrome c oxidase IEndangered speciesNext-generation sequencingWildlife forensic samples

Identifiers

PMID27178552
PMCPMC4909793

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.