Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans under low temperature.

Fei Yin, Peng Sun, Jiteng Wang, Quanxin Gao

Abstract read

In one paragraph

Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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6citing papers in PubMed

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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

Honokiol induces apoptosis-like death in Cryptocaryon irritans Tomont.Parasites & vectors · 2023
Article
Transcriptome Analysis ofGenes · 2020
Article
A new age in AquaMedicine: unconventional approach in studying aquatic diseases.BMC veterinary research · 2018
Review
Molecular mechanisms of an antimicrobial peptide piscidin (Lc-pis) in a parasitic protozoan, Cryptocaryon irritans.BMC genomics · 2018
Article
Transcriptome Analysis Based on RNA-Seq in Understanding Pathogenic Mechanisms of Diseases and the Immune System of Fish: A Comprehensive Review.International journal of molecular sciences · 2018
Review
Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress.PloS one · 2017
Article

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5 · Who and what money

Authors and funding

4 authors.

Fei YinKey Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Room 316, Building 6, 300 Jungong Road, Shanghai, 200090, PR China. feige895@gmail.com.

Peng SunKey Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Room 316, Building 6, 300 Jungong Road, Shanghai, 200090, PR China.

Jiteng WangFisheries College of Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, PR China.

Quanxin GaoKey Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Room 316, Building 6, 300 Jungong Road, Shanghai, 200090, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCryptocaryon irritans, a species of obligatory ciliate ectoparasite, can infect various species of marine teleost fish. Cryptocaryon irritans that fall to the seabed or aquarium bottom in winter can form "dormant tomonts" and wake up when the temperature rises the next year. Abundant studies and analyses on the dormant tomonts were carried out at the transcriptome level, in order to investigate the molecular mechanism of C. irritans tomonts entering the dormant state under low-temperature conditions.

methodsThe paired-end sequencing strategy was used to better assemble the entire transcriptome de novo. All clean sequencing reads from each of the three libraries (Group A: untreated blank control; Group B: treated for 24 h at 12 °C; and Group C: developed for 24 h at 25 °C) were respectively mapped back to the transcriptome assembly using the bioinformatics software.

resultsIn this study, 25,695,034, 21,944,467, and 28,722,875 paired-end clean reads were obtained respectively from the three cDNA libraries of the C. irritans tomont by Illumina paired-end sequencing technology. A total of 25,925 unique transcript fragments (unigenes) were assembled, with an average length of 839 bp. Differentially expressed genes (DEGs) were scrutinized; in Group B/A pairwise comparison, 343 genes presented differential expression, including 265 up-regulated genes and 78 down-regulated genes in Group B; in Group C/A pairwise comparison, there were 567 DEGs, including 548 up-regulated genes and 19 down-regulated genes in Group C; and in Group B/C pairwise comparison, 185 genes showed differential expression, including 145 up-regulated genes and 40 down-regulated genes in Group B.

conclusionsThis is the first transcriptomic analytical study of the C. irritans tomonts under low temperature. It can be concluded that most of the genes required for its cell survival under low temperature, or for cell entry into a deeper dormancy state were discovered, and that they might be considered as candidate genes to develop the diagnostic and control measures for cryptocaryoniasis.

Indexed as

Cold TemperatureTranscriptomeAnimalsCiliophoraCiliophora InfectionsFish DiseasesFishesGene Expression ProfilingGene LibraryGene OntologySequence Analysis, RNACell divisionCryptocaryon irritansDormant tomontLow temperatureTranscriptome

Identifiers

PMID27177617

DOI10.1186/s13071-016-1550-1

PMCPMC4867990

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