ArticleBiological research2016
Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer.
Article in Biological research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Long Non-Coding RNAs and RNA-Binding Proteins in Pancreatic Cancer Development and Progression.Cancers · 2025Review
- Retraction Note: Downregulation of CDKL1 suppresses neuroblastoma cell proliferation, migration and invasion.Cellular & molecular biology letters · 2025Article
- Cancer and Alzheimer's Inverse Correlation: an Immunogenetic Analysis.Molecular neurobiology · 2023Review
- Article
- Proteomic analysis enables distinction of early- versus advanced-stage lung adenocarcinomas.Clinical and translational medicine · 2020Article
- Oxytocin and oxytocin receptor alterations, decreased survival, and increased chemoresistance in patients with pancreatic cancer.Hepatobiliary & pancreatic diseases international : HBPD INT · 2020Article
- Genetic association and functional characterization of MCPH1 gene variation in bipolar disorder and schizophrenia.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2019Article
- Genome-wide association study identifies QTLs for displacement of abomasum in Chinese Holstein cattle1.Journal of animal science · 2019Article
- Exosomes impact survival to radiation exposure in cell line models of nervous system cancer.Oncotarget · 2018Article
- Expression of Concern to: Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer.Biological research · 2018Article
- ZFR coordinates crosstalk between RNA decay and transcription in innate immunity.Nature communications · 2018Article
- Retracted: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex.BioMed research international · 2017Article
- Zinc finger RNA-binding protein promotes non-small-cell carcinoma growth and tumor metastasis by targeting the Notch signaling pathway.American journal of cancer research · 2017Article
Corrections and comments
- Expression of concern · 2018-06-22Concerns/Issues about Authorship/Affiliation · Concerns/Issues about Data · Concerns/Issues about Referencing/Attributions ·
- Expression of concern
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundZinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer.
methodsHerein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expression in human pancreatic tumors. Then short hairpin RNA (shRNA) targeting ZFR was designed and delivered into PANC-1 pancreatic cancer cells to knock down ZFR expression. Cell viability, cell proliferation and cell cycle analysis after ZFR knockdown were determined by MTT, colony forming and FACS, respectively. In addition, cell migration and invasion were assessed using the Transwell system.
resultsThe expression of ZFR was significantly higher in pancreatic tumors than normal pancreas tissues by Oncomine database analysis. Knockdown of ZFR by shRNA-expressing lentivirus significantly decreased the viability and invasion ability of pancreatic cancer cells. Moreover, FACS analysis showed that knockdown of ZFR in PANC-1 cells caused a significant cell cycle arrest at G0/G1 phase. Furthermore, knockdown of ZFR decreased the levels of CDK2, CDK4, CyclinA and CyclinD1 and enhanced the expression of p27, which has evidenced by qRT-PCR and Western blot analysis.
conclusionsKnockdown of ZFR might provide a novel alternative to targeted therapy of pancreatic cancer and deserves further investigation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.