Evidence map›Paper›PMID 27177577›Full record

ArticleStem cells translational medicine2016

Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium.

Beth A Lindborg, John H Brekke, Amanda L Vegoe, Connor B Ulrich, Kerri T Haider, Sandhya Subramaniam, Scott L Venhuizen, Cindy R Eide, Paul J Orchard, Weili Chen and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed, 2 pooled it
7.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 2 syntheses or guidelines pooled it, 142 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Understanding the Molecular Basis of Miller-Dieker Syndrome.International journal of molecular sciences · 2025
    Review
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  10. Article
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  12. Article
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  17. Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  18. Review
  19. Review
  20. Review

16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 1 institution in 1 country.

Beth A LindborgStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Bioactive Regenerative Therapeutics, Inc., Two Harbors, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA.
John H BrekkeBioactive Regenerative Therapeutics, Inc., Two Harbors, Minnesota, USA.
Amanda L VegoeStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Connor B UlrichStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Kerri T HaiderStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Sandhya SubramaniamStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA Department of Neurosurgery, University of Minnesota, Minneapolis, Minnesota, USA.
Scott L VenhuizenStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Cindy R EideStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.
Paul J OrchardDepartment of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.
Weili ChenStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.
Qi WangBiostatistical Design and Analysis Center, Clinical and Translational Science Institute, University of Minnesota, Minneapolis, Minnesota, USA.
Francisco PelaezDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Carolyn M ScottDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Efrosini KokkoliDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Susan A KeirsteadStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Integrative Biology and Physiology, University of Minnesota, Minneapolis, Minnesota, USA.
James R DuttonStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Jakub TolarStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.
Timothy D O'BrienStem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA obrie004@umn.edu.
University of Minnesota · US

Funding

Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Douglas Yee · 1998 to 2026
$100.4M
University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UL1TR002494 · NCATS · UNIVERSITY OF MINNESOTA · PI BLAZAR, BRUCE R, WEISDORF, DANIEL J · 2018 to 2022
$34.9M
NCATS NIH HHS UL1 TR002494NCI NIH HHS P30 CA077598
6 · The paper itself

Abstract

unlabelledTissue organoids are a promising technology that may accelerate development of the societal and NIH mandate for precision medicine. Here we describe a robust and simple method for generating cerebral organoids (cOrgs) from human pluripotent stem cells by using a chemically defined hydrogel material and chemically defined culture medium. By using no additional neural induction components, cOrgs appeared on the hydrogel surface within 10-14 days, and under static culture conditions, they attained sizes up to 3 mm in greatest dimension by day 28. Histologically, the organoids showed neural rosette and neural tube-like structures and evidence of early corticogenesis. Immunostaining and quantitative reverse-transcription polymerase chain reaction demonstrated protein and gene expression representative of forebrain, midbrain, and hindbrain development. Physiologic studies showed responses to glutamate and depolarization in many cells, consistent with neural behavior. The method of cerebral organoid generation described here facilitates access to this technology, enables scalable applications, and provides a potential pathway to translational applications where defined components are desirable. SIGNIFICANCE: Tissue organoids are a promising technology with many potential applications, such as pharmaceutical screens and development of in vitro disease models, particularly for human polygenic conditions where animal models are insufficient. This work describes a robust and simple method for generating cerebral organoids from human induced pluripotent stem cells by using a chemically defined hydrogel material and chemically defined culture medium. This method, by virtue of its simplicity and use of defined materials, greatly facilitates access to cerebral organoid technology, enables scalable applications, and provides a potential pathway to translational applications where defined components are desirable.

Indexed as

Biomechanical PhenomenaBrainCell DifferentiationCells, CulturedCulture MediaGene ExpressionHumansHydrogel, Polyethylene Glycol DimethacrylateInduced Pluripotent Stem CellsNeuronsOrganoidsTissue Culture TechniquesCulture MediaHydrogel, Polyethylene Glycol DimethacrylateAdrenoleukodystrophyBrainIn vitro techniquesOrganoidsStem cells

Identifiers

PMID27177577
PMCPMC4922855
OpenAlexW2369036439

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.