ArticleBMC biotechnology2016
Genomic variation and DNA repair associated with soybean transgenesis: a comparison to cultivars and mutagenized plants.
Article in BMC biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Deciphering the complex molecular architecture of the genetically modified soybean FG72 through paired-end whole genome sequencing.Food chemistry. Molecular sciences · 2025Article
- Soybean genomics research community strategic plan: A vision for 2024-2028.The plant genome · 2024Article
- A strategy for identification and characterization of genic mutations using a temperature-sensitive chlorotic soybean mutant as an example.Plant direct · 2024Article
- Agrobacterium-mediated transfer of the Fusarium graminearum Tri6 gene into barley using mature seed-derived shoot tips as explants.Plant cell reports · 2024Article
- Fast neutron mutagenesis in soybean enriches for small indels and creates frameshift mutations.G3 (Bethesda, Md.) · 2022Article
- Advances in CRISPR/Cas9.BioMed research international · 2022Review
- Influence of transgenesis on genome variability in cucumber lines with aPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Plant Genome Editing and the Relevance of Off-Target Changes.Plant physiology · 2020Article
- Genome editing with the CRISPR-Cas system: an art, ethics and global regulatory perspective.Plant biotechnology journal · 2020Review
- Genomic variation between PRSV resistant transgenic SunUp and its progenitor cultivar Sunset.BMC genomics · 2020Article
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
- Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement.Plants (Basel, Switzerland) · 2019Review
- CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement.Scientific reports · 2019Article
- The evolving EU regulatory framework for precision breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- NGS sequencing reveals that many of the genetic variations in transgenic rice plants match the variations found in natural rice population.Genes & genomics · 2019Article
- Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize.The Plant cell · 2019Article
- Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?Frontiers in plant science · 2019Review
- Proposed U.S. regulation of gene-edited food animals is not fit for purpose.NPJ science of food · 2019Review
- Familiarity in the Context of Risk Assessment of Transgenic Crops: Focus on Some Countries in the Americas.Frontiers in bioengineering and biotechnology · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods.
resultsOn average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms.
conclusionsOverall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
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