ArticleNeurophotonics2016
Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome.
Article in Neurophotonics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Comparative connectomics reveals stage-specific gap junction rewiring that reshapes avoidance behavior.bioRxiv : the preprint server for biology · 2026Article
- Visualizing Intracellular Localization of Natural-Product-Based Chemical Probes Using Click-Correlative Light and Electron Microscopy.ACS chemical biology · 2025Article
- Array tomography: trails to discovery.Methods in microscopy · 2024Article
- Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit.eLife · 2024Article
- Studying zebrafish nervous system structure and function in health and disease with electron microscopy.Development, growth & differentiation · 2023Review
- Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy.eLife · 2022Article
- Three-dimensional SEM, TEM, and STEM for analysis of large-scale biological systems.Histochemistry and cell biology · 2022Review
- Visualizing Presynaptic Active Zones and Synaptic Vesicles.Frontiers in synaptic neuroscience · 2022Article
- Surface charge manipulation and electrostatic immobilization of synaptosomes for super-resolution imaging: a study on tau compartmentalization.Scientific reports · 2021Article
- Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy.Frontiers in cell and developmental biology · 2021Article
- Overexpression of an ALS-associated FUS mutation inBiology open · 2020Article
- Registration and Visualization of Correlative Super-Resolution Microscopy Data.Biophysical journal · 2019Article
- Comparative Principles for Next-Generation Neuroscience.Frontiers in behavioral neuroscience · 2019Article
- Q&A: Array tomography.BMC biology · 2018Article
- A Pipeline for Volume Electron Microscopy of theFrontiers in neural circuits · 2018Article
- Article
- Inducing fluorescence of uranyl acetate as a dual-purpose contrast agent for correlative light-electron microscopy with nanometre precision.Scientific reports · 2017Article
- FIJI Macro 3D ART VeSElecT: 3D Automated Reconstruction Tool for Vesicle Structures of Electron Tomograms.PLoS computational biology · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Correlating molecular labeling at the ultrastructural level with high confidence remains challenging. Array tomography (AT) allows for a combination of fluorescence and electron microscopy (EM) to visualize subcellular protein localization on serial EM sections. Here, we describe an application for AT that combines near-native tissue preservation via high-pressure freezing and freeze substitution with super-resolution light microscopy and high-resolution scanning electron microscopy (SEM) analysis on the same section. We established protocols that combine SEM with structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (dSTORM). We devised a method for easy, precise, and unbiased correlation of EM images and super-resolution imaging data using endogenous cellular landmarks and freely available image processing software. We demonstrate that these methods allow us to identify and label gap junctions in Caenorhabditis elegans with precision and confidence, and imaging of even smaller structures is feasible. With the emergence of connectomics, these methods will allow us to fill in the gap-acquiring the correlated ultrastructural and molecular identity of electrical synapses.
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