Evidence map›Paper›PMID 27175366›Full record

ArticleAorta (Stamford, Conn.)2015

Increased Expression of Lamin A/C Correlate with Regions of High Wall Stress in Abdominal Aortic Aneurysms.

Amir Malkawi, Grisha Pirianov, Evelyn Torsney, Ian Chetter, Natzi Sakalihasan, Ian M Loftus, Ian Nordon, Christopher Huggins, Nicoletta Charolidi, Matt Thompson and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aorta (Stamford, Conn.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Evidence for Drop-Like Nuclear Deformation in Sheared Endothelial Monolayers.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  2. Abdominal aortic aneurysms.Nature reviews. Disease primers · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Amir MalkawiDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Grisha PirianovDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Evelyn TorsneyDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Ian ChetterCentre for Cardiovascular & Metabolic Research, York Hull Medical School, Hull, UK.
Natzi SakalihasanDepartment of Cardiovascular Surgery, University Hospital of Liege, Liege, Belgium.
Ian M LoftusDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Ian NordonDepartment of Vascular Surgery, University Hospital Southampton, Southampton, UK.
Christopher HugginsDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Nicoletta CharolidiDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Matt ThompsonDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Xie Yun XuDepartment of Chemical Engineering, Imperial College London, London, UK.
Gillian W CockerillDepartment of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK.
Anglia Ruskin University · GBHull York Medical School · GBImperial College London · GBUniversity Hospital Southampton NHS Foundation Trust · GBUniversity of Liège · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSince aortic diameter is the most -significant risk factor for rupture, we sought to identify stress-dependent changes in gene expression to illuminate novel molecular processes in aneurysm rupture. MATERIALS AND

methodsWe constructed finite element maps of abdominal computerized tomography scans (CTs) of seven abdominal aortic aneurysm (AAA) patients to map wall stress. Paired biopsies from high- and low-stress areas were collected at surgery using vascular landmarks as coordinates. Differential gene expression was evaluated by Illumina Array analysis, using the whole genome DNA-mediated, annealing, selection, extension, and ligation (DASL) gene chip (n = 3 paired samples).

resultsThe sole significant candidate from this analysis, Lamin A/C, was validated at the protein level, using western blotting. Lamin A/C expression in the inferior mesenteric vein (IMV) of AAA patients was compared to a control group and in aortic smooth muscle cells in culture in response to physiological pulsatile stretch. -Areas of high wall stress (n = 7) correlate to those -regions which have the thinnest walls [778 µm (585-1120 µm)] in comparison to areas of lowest wall stress [1620 µm (962-2919 µm)]. Induced expression of Lamin A/C -correlated with areas of high wall stress from AAAs but was not significantly induced in the IMV from AAA patients compared to controls (n = 16). Stress-induced expression of Lamin A/C was mimicked by exposing aortic smooth muscle cells to prolonged pulsatile stretch.

conclusionLamin A/C protein is specifically increased in areas of high wall stress in AAA from patients, but is not increased on other vascular beds of aneurysm patients, suggesting that its elevation may be a compensatory response to the pathobiology leading to aneurysms.

Indexed as

AgingNuclear laminaSmooth muscle cellsStretch

Identifiers

PMID27175366
PMCPMC4851850
OpenAlexW2344760397

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.