Evidence map›Paper›PMID 27175198›Full record

ArticleIranian journal of veterinary research2015

Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan.

U Farooq, A Latif, H Irshad, A Ullah, A B Zahur, K Naeem, S U H Khan, Z Ahmed, L L Rodriguez, G Smoliga

Abstract read
In one paragraph

Article in Iranian journal of veterinary research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Advances in the Diagnosis of Foot-and-Mouth Disease.Frontiers in veterinary science · 2020
    Review
  7. Amplification chemistries in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2019
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

U FarooqAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
A LatifAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
H IrshadAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
A UllahAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
A B ZahurAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
K NaeemAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
S U H KhanAnimal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan;
Z AhmedPlum Island Animal Disease Centre, Foreign Animal Disease Research Unit, New York 11944, USA.
L L RodriguezPlum Island Animal Disease Centre, Foreign Animal Disease Research Unit, New York 11944, USA.
G SmoligaPlum Island Animal Disease Centre, Foreign Animal Disease Research Unit, New York 11944, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limitations of lateral flow devices. Therefore, a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) was standardized using universal and sero-type specific genes in a single tube. This test does not require sophisticated equipment and can detect FMDV at serotype level in about 60 min. In addition, the sensitivity and specificity of this test is comparable to conventional reverse transcriptase PCR and real time PCR (rRT-PCR).

Indexed as

FMDRT-LAMPSensitivity

Identifiers

PMID27175198
PMCPMC4782670

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.