ArticleNucleic acids research2016
HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Microfluidic-Assisted Evolution of a Robust NADChemSusChem · 2025Article
- Structural and Functional Characterization of Obesumbacterium proteus Phytase: A Comprehensive In-Silico Study.Molecular biotechnology · 2025Article
- Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins.Frontiers in plant science · 2025Review
- Enriching productive mutational paths accelerates enzyme evolution.Nature chemical biology · 2024Article
- Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system.Nature communications · 2024Article
- The Improved Antineoplastic Activity of Thermophilic L-Asparaginase Tli10209 via Site-Directed Mutagenesis.Biomolecules · 2024Article
- Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst.Frontiers in bioengineering and biotechnology · 2024Article
- Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases' Thermostability.International journal of molecular sciences · 2023Review
- LibGENiE - A bioinformatic pipeline for the design of information-enriched enzyme libraries.Computational and structural biotechnology journal · 2023Article
- SoluProtMutComputational and structural biotechnology journal · 2022Article
- Current state of and need for enzyme engineering of 2-deoxy-D-ribose 5-phosphate aldolases and its impact.Applied microbiology and biotechnology · 2021Review
- Recent advances in user-friendly computational tools to engineer protein function.Briefings in bioinformatics · 2021Review
- Synthesis of libraries and multi-site mutagenesis using a PCR-derived, dU-containing template.Synthetic biology (Oxford, England) · 2021Article
- Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods.Applied microbiology and biotechnology · 2020Article
- Practically useful protein-design methods combining phylogenetic and atomistic calculations.Current opinion in structural biology · 2020Review
- Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae.Scientific reports · 2020Article
- Distant Non-Obvious Mutations Influence the Activity of a HyperthermophilicBiomolecules · 2019Article
- A molecular simulation analysis of vitamin D targets interleukin 13 (IL13) as an alternative to mometasone in asthma.3 Biotech · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart libraries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evolutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four different protein engineering strategies and optimizing the resulting library's size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein's structure and visualized with a JSmol applet. HotSpot Wizard lists annotated residues suitable for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive annotations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries. It is freely available at http://loschmidt.chemi.muni.cz/hotspotwizard.
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