ArticleScientific reports2016
Plant phosphomannose isomerase as a selectable marker for rice transformation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.3 Biotech · 2025Review
- Sugar alcohols in plants: implications for enhancing tree seedlings drought tolerance and production strategies.BMC plant biology · 2025Review
- Conditional knockdown of OsMLH1 to improve plant prime editing systems without disturbing fertility in rice.Genome biology · 2024Article
- Article
- Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI).Transgenic research · 2023Article
- Glucomannan inGenes · 2022Review
- Development of a highly efficient prime editor 2 system in plants.Genome biology · 2022Article
- Advances in gene editing without residual transgenes in plants.Plant physiology · 2022Article
- Genome editing with type II-C CRISPR-Cas9 systems from Neisseria meningitidis in rice.Plant biotechnology journal · 2022Article
- Comparing soil-to-plant cadmium (Cd) transfer and potential human intake among rice cultivars with different Cd tolerance levels grown in a tropical contaminated soil.Environmental monitoring and assessment · 2021Article
- Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato.Plant diversity · 2021Article
- Article
- Development of Plant Prime-Editing Systems for Precise Genome Editing.Plant communications · 2020Article
- How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis.Rice (New York, N.Y.) · 2020Review
- Plant-associated and soil microbiota composition as a novel criterion for the environmental risk assessment of genetically modified plants.GM crops & food · 2020Article
- Molecular and Toxicity Analyses of White Granulated Sugar and Other Processing Products Derived From Transgenic Sugarcane.Frontiers in plant science · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Efficient Biolistic Transformation of Immature Citrus Rootstocks Using Phosphomannose-isomerase Selection.Plants (Basel, Switzerland) · 2019Article
- Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019Review
- Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The E. coli phosphomannose isomerase (EcPMI) gene is widely used as a selectable marker gene (SMG) in mannose (Man) selection-based plant transformation. Although some plant species exhibit significant PMI activity and active PMIs were even identified in Man-sensitive plants, whether plant PMIs can be used as SMGs remains unclear. In this study, we isolated four novel PMI genes from Chlorella variabilis and Oryza sativa. Their isoenzymatic activities were examined in vitro and compared with that of EcPMI. The active plant PMIs were separately constructed into binary vectors as SMGs and then transformed into rice via Agrobacterium. In both Indica and Japonica subspecies, our results indicated that the plant PMIs could select and produce transgenic plants in a pattern similar to that of EcPMI. The transgenic plants exhibited an accumulation of plant PMI transcripts and enhancement of the in vivo PMI activity. Furthermore, a gene of interest was successfully transformed into rice using the plant PMIs as SMGs. Thus, novel SMGs for Man selection were isolated from plants, and our analysis suggested that PMIs encoding active enzymes might be common in plants and could potentially be used as appropriate genetic elements in cisgenesis engineering.
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