ArticleMetabolic engineering2016
Evidence for transketolase-like TKTL1 flux in CHO cells based on parallel labeling experiments and (13)C-metabolic flux analysis.
Article in Metabolic engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A Stable Isotope Tracing Primer for the Mass Spectrometrist.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Structural and Computational Insights into Transketolase-like 1 (TKTL-1): Distinction from TKT and Implications for Cancer Metabolism and Therapeutic Targeting.Molecules (Basel, Switzerland) · 2025Article
- Comprehensive stable-isotope tracing of glucose and amino acids identifies metabolic by-products and their sources in CHO cell culture.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Into the metabolic wild: Unveiling hidden pathways of microbial metabolism.Microbial biotechnology · 2024Article
- Interpreting metabolic complexity via isotope-assisted metabolic flux analysis.Trends in biochemical sciences · 2023Review
- Article
- Isotopic Tracer for Absolute Quantification of Metabolites of the Pentose Phosphate Pathway in Bacteria.Metabolites · 2022Review
- Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas.RSC advances · 2022Review
- Hot isopropanol quenching procedure for automated microtiter plate scaleMicrobial cell factories · 2022Article
- Quantitative metabolic fluxes regulated by trans-omic networks.The Biochemical journal · 2022Review
- Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed-batch process.Biotechnology and bioengineering · 2022Article
- Cell culture bioprocessing - the road taken and the path forward.Current opinion in chemical engineering · 2020Article
- Computational data mining method for isotopomer analysis in the quantitative assessment of metabolic reprogramming.Scientific reports · 2020Article
- High-resolutionNature protocols · 2019Article
- Tandem Mass Spectrometry forFrontiers in microbiology · 2019Article
- Hexokinase-2 depletion inhibits glycolysis and induces oxidative phosphorylation in hepatocellular carcinoma and sensitizes to metformin.Nature communications · 2018Article
- Mass Spectrometry-Based Method to Study Inhibitor-Induced Metabolic Redirection in the Central Metabolism of Cancer Cells.Mass spectrometry (Tokyo, Japan) · 2018Article
- Tracing insights into human metabolism using chemical engineering approaches.Current opinion in chemical engineering · 2016Article
- Article
- Comprehensive metabolic modeling of multiple 13C-isotopomer data sets to study metabolism in perfused working hearts.American journal of physiology. Heart and circulatory physiology · 2016Article
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3 authors.
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Abstract
The pentose phosphate pathway (PPP) is a fundamental component of cellular metabolism. It provides precursors for the biosynthesis of nucleotides and contributes to the production of reducing power in the form of NADPH. It has been hypothesized that mammalian cells may contain a hidden reaction in PPP catalyzed by transketolase-like protein 1 (TKTL1) that is closely related to the classical transketolase enzyme; however, until now there has been no direct experimental evidence for this reaction. In this work, we have applied state-of-the-art techniques in (13)C metabolic flux analysis ((13)C-MFA) based on parallel labeling experiments and integrated flux fitting to estimate the TKTL1 flux in CHO cells. We identified a set of three parallel labeling experiments with [1-(13)C]glucose+[4,5,6-(13)C]glucose, [2-(13)C]glucose+[4,5,6-(13)C]glucose, and [3-(13)C]glucose+[4,5,6-(13)C]glucose and developed a new method to measure (13)C-labeling of fructose 6-phosphate by GC-MS that allows intuitive interpretation of mass isotopomer distributions to determine key fluxes in the model, including glycolysis, oxidative PPP, non-oxidative PPP, and the TKTL1 flux. Using these tracers we detected a significant TKTL1 flux in CHO cells at the stationary phase. The flux results suggest that the main function of oxidative PPP in CHO cells at the stationary phase is to fuel the TKTL1 reaction. Overall, this study demonstrates for the first time that carbon atoms can be lost in the PPP, by means other than the oxidative PPP, and that this loss of carbon atoms is consistent with the hypothesized TKTL1 reaction in mammalian cells.
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