Evidence map›Paper›PMID 27174199›Full record

ArticleScientific reports2016

Macrophage phagocytosis alters the MRI signal of ferumoxytol-labeled mesenchymal stromal cells in cartilage defects.

Hossein Nejadnik, Olga Lenkov, Florian Gassert, Deborah Fretwell, Isaac Lam, Heike E Daldrup-Link

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02893293 (Imaging of Osteonecrosis With Ferumoxytol-Enhanced MRI), which is not on this map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02893293 phase4completednot on this map

Imaging of Osteonecrosis With Ferumoxytol-Enhanced MRI

TypeinterventionalSponsorStanford UniversityRan2015 to 2023Enrolled14ConditionsOsteonecrosisArmsFerumoxytol-enhanced magnetic resonance imaging, Magentic Resonance Imaging
3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hossein NejadnikDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Olga LenkovDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Florian GassertDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Deborah FretwellDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Isaac LamDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Heike E Daldrup-LinkDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.

Funding

Monitoring of Stem Cell Engraftment in Arthritic Joints with MR ImagingR01AR054458 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DALDRUP-LINK, HEIKE ELIZABETH · 2008 to 2021
$6.7M
NIAMS NIH HHS R01 AR054458
6 · The paper itself

Abstract

Human mesenchymal stem cells (hMSCs) are a promising tool for cartilage regeneration in arthritic joints. hMSC labeling with iron oxide nanoparticles enables non-invasive in vivo monitoring of transplanted cells in cartilage defects with MR imaging. Since graft failure leads to macrophage phagocytosis of apoptotic cells, we evaluated in vitro and in vivo whether nanoparticle-labeled hMSCs show distinct MR signal characteristics before and after phagocytosis by macrophages. We found that apoptotic nanoparticle-labeled hMSCs were phagocytosed by macrophages while viable nanoparticle-labeled hMSCs were not. Serial MRI scans of hMSC transplants in arthritic joints of recipient rats showed that the iron signal of apoptotic, nanoparticle-labeled hMSCs engulfed by macrophages disappeared faster compared to viable hMSCs. This corresponded to poor cartilage repair outcomes of the apoptotic hMSC transplants. Therefore, rapid decline of iron MRI signal at the transplant site can indicate cell death and predict incomplete defect repair weeks later. Currently, hMSC graft failure can be only diagnosed by lack of cartilage defect repair several months after cell transplantation. The described imaging signs can diagnose hMSC transplant failure more readily, which could enable timely re-interventions and avoid unnecessary follow up studies of lost transplants.

Indexed as

AnimalsCartilage DiseasesCell SurvivalContrast MediaDisease Models, AnimalFerrosoferric OxideHumansMacrophagesMagnetic Resonance ImagingMagnetite NanoparticlesMesenchymal Stem CellsMesenchymal Stem Cell TransplantationPhagocytosisRatsTHP-1 CellsContrast MediaFerrosoferric OxideMagnetite Nanoparticles

Identifiers

PMID27174199
PMCPMC4865731

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.