ArticleScientific reports2016
Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Abnormal zona pellucida and follicular development inZoological research · 2025Article
- Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Pancreatic agenesis and altered m6A methylation in the pancreas of PDX1-mutant cynomolgus macaques.Zoological research · 2024Article
- CRISPR/Cas9 genome editing to create nonhuman primate models for studying stem cell therapies for HIV infection.Retrovirology · 2022Review
- First Successful Delivery after Uterus Transplantation in MHC-Defined Cynomolgus Macaques.Journal of clinical medicine · 2020Article
- Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos.Scientific reports · 2020Article
- Multiplex precise base editing in cynomolgus monkeys.Nature communications · 2020Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The efficiency of assisted reproductive technologies (ARTs) in nonhuman primates is low due to no screening criterions for selecting sperm, oocyte, and embryo as well as its surrogate mothers. Here we analyzed 15 pairs of pregnant and non-pregnant cynomolgus monkeys, each pair of which received embryos from one batch of fertilized oocytes, and found ratio of endometrial to myometrial thicknesses in abdominal ultrasonic transverse section of uterus is a reliable indicator for selection of recipients for embryo transfer. We performed 305 ovarian stimulations in 128 female cynomolgus monkeys and found that ovarian stimulation can be performed in a whole year and repeated up to six times in the same monkey without deteriorating fertilization potential of eggs until a poor response to stimulation happened. Fertilization can be efficiently achieved with both conventional and piezo-driven intracytoplasmic sperm injection procedures. In semen collection, semen quality is higher with the penile robe electrical stimulus method compared with the rectal probe method. Moreover, caesarean section is an effective strategy for increasing baby survival rates of multiple pregnancies. These findings provide a practical guidance for the efficient use of ARTs, facilitating their use in genetic engineering of macaque monkeys for basic and translational neuroscience research.
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Registered trials
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