ArticleNature protocols2016
Radiolabeling of DOTA-like conjugated peptides with generator-produced (68)Ga and using NaCl-based cationic elution method.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- ScalableWorld journal of nuclear medicine · 2026Article
- Extending the shelf life of ⁶⁸Ge/⁶⁸Ga generators via preconcentration of [⁶⁸Ga]GaCl₃ for preclinical application.EJNMMI radiopharmacy and chemistry · 2025Article
- Synthesis of DOTA-BasedMethods and protocols · 2025Article
- Specific reaction conditions for efficient automatedFrontiers in medicine · 2025Article
- Simultaneous Positron Emission Tomography and Molecular Magnetic Resonance Imaging of Cardiopulmonary Fibrosis in a Mouse Model of Left Ventricular Dysfunction.Journal of the American Heart Association · 2024Article
- Development and application of a fully automatic multi-function cassette module Mortenon M1 for radiopharmaceutical synthesis.Annals of nuclear medicine · 2024Article
- Production and purification ofScientific reports · 2023Article
- Microfluidic-based production of [EJNMMI radiopharmacy and chemistry · 2023Article
- Detection of Pulmonary Fibrosis with a Collagen-Mimetic Peptide.ACS sensors · 2023Article
- [Pharmaceutics · 2023Article
- Radiolabeled nanomaterial for cancer diagnostics and therapeutics: principles and concepts.Cancer nanotechnology · 2023Review
- Cyclotron Production of Gallium-68 Radiopharmaceuticals Using thePharmaceutics · 2022Review
- [International journal of molecular sciences · 2022Article
- Evaluation ofDiagnostics (Basel, Switzerland) · 2022Article
- Good practices forEJNMMI radiopharmacy and chemistry · 2022Review
- Evaluation of a Radiolabeled Macrocyclic Peptide as Potential PET Imaging Probe for PD-L1.ChemMedChem · 2022Article
- Novel Approaches to Imaging the Pulmonary Vasculature and Right Heart.Circulation research · 2022Review
- Diagnostic Value of Radiolabelled Somatostatin Analogues for Neuroendocrine Tumour Diagnosis: The Benefits and Drawbacks of [Cancers · 2022Review
- Feasibility, Biodistribution, and Preliminary Dosimetry in Peptide-Targeted Radionuclide Therapy of Diverse Adenocarcinomas UsingJournal of nuclear medicine : official publication, Society of Nuclear Medicine · 2022Article
- High-Efficiency Cationic Labeling Algorithm of Macroaggregated Albumin withNuclear medicine and molecular imaging · 2021Article
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8 authors.
Funding
Abstract
Gallium-68 ((68)Ga) is a generator-produced radionuclide with a short half-life (t½ = 68 min) that is particularly well suited for molecular imaging by positron emission tomography (PET). Methods have been developed to synthesize (68)Ga-labeled imaging agents possessing certain drawbacks, such as longer synthesis time because of a required final purification step, the use of organic solvents or concentrated hydrochloric acid (HCl). In our manuscript, we provide a detailed protocol for the use of an advantageous sodium chloride (NaCl)-based method for radiolabeling of chelator-modified peptides for molecular imaging. By working in a lead-shielded hot-cell system,(68)Ga(3+) of the generator eluate is trapped on a cation exchanger cartridge (100 mg, ∼8 mm long and 5 mm diameter) and then eluted with acidified 5 M NaCl solution directly into a sodium acetate-buffered solution containing a DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) or DOTA-like chelator-modified peptide. The main advantages of this procedure are the high efficiency and the absence of organic solvents. It can be applied to a variety of peptides, which are stable in 1 M NaCl solution at a pH value of 3-4 during reaction. After labeling, neutralization, sterile filtration and quality control (instant thin-layer chromatography (iTLC), HPLC and pH), the radiopharmaceutical can be directly administered to patients, without determination of organic solvents, which reduces the overall synthesis-to-release time. This procedure has been adapted easily to automated synthesis modules, which leads to a rapid preparation of (68)Ga radiopharmaceuticals (12-16 min).
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