Evidence map›Paper›PMID 27171472›Full record

ArticlePloS one2016

Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.

Yan Holtz, Morgane Ardisson, Vincent Ranwez, Alban Besnard, Philippe Leroy, Gérard Poux, Pierre Roumet, Véronique Viader, Sylvain Santoni, Jacques David

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
6.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

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  17. Epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017
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  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Yan HoltzMontpellier SupAgro, UMR AGAP, Montpellier, France.
Morgane ArdissonINRA, UMR AGAP, Montpellier, France.
Vincent RanwezMontpellier SupAgro, UMR AGAP, Montpellier, France.
Alban BesnardINRA, UMR AGAP, Montpellier, France.
Philippe LeroyINRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont Ferrand, France.
Gérard PouxINRA, UMR AGAP, Montpellier, France.
Pierre RoumetINRA, UMR AGAP, Montpellier, France.
Véronique ViaderINRA, UMR AGAP, Montpellier, France.
Sylvain SantoniINRA, UMR AGAP, Montpellier, France.
Jacques DavidMontpellier SupAgro, UMR AGAP, Montpellier, France.
Agropolis International · FRInstitut Agro Montpellier · FRInstitut National de la Recherche Agronomique · MA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeted sequence capture is a promising technology which helps reduce costs for sequencing and genotyping numerous genomic regions in large sets of individuals. Bait sequences are designed to capture specific alleles previously discovered in parents or reference populations. We studied a set of 135 RILs originating from a cross between an emmer cultivar (Dic2) and a recent durum elite cultivar (Silur). Six thousand sequence baits were designed to target Dic2 vs. Silur polymorphisms discovered in a previous RNAseq study. These baits were exposed to genomic DNA of the RIL population. Eighty percent of the targeted SNPs were recovered, 65% of which were of high quality and coverage. The final high density genetic map consisted of more than 3,000 markers, whose genetic and physical mapping were consistent with those obtained with large arrays.

Indexed as

AllelesChromosome MappingContig MappingGenotyping TechniquesPolymorphism, GeneticPolymorphism, Single NucleotideSequence Analysis, DNATriticum

Identifiers

PMID27171472
PMCPMC4865223
OpenAlexW2368501952

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.