ArticlePLoS genetics2016
Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 54 citations in OpenAlex.
- Topo IV is required to allow replisomes to converge and complete replication on the chromosome.PLoS genetics · 2025Article
- Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structure.Nature communications · 2025Article
- The pathway to resolve dimeric forms distinguishes plasmids from megaplasmids in Enterobacteriaceae.Nucleic acids research · 2025Article
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- In Vitro and In Silico Biological Activities Investigation of Ethyl Acetate Extract ofPlants (Basel, Switzerland) · 2024Article
- Temporospatial control of topoisomerases by essential cellular processes.Current opinion in microbiology · 2024Review
- DciA secures bidirectional replication initiation in Vibrio cholerae.Nucleic acids research · 2024Article
- The role of prokaryotic argonautes in resistance to type II topoisomerases poison ciprofloxacin.Biochemical Society transactions · 2024Review
- Highly sensitive mapping of in vitro type II topoisomerase DNA cleavage sites with SHAN-seq.Nucleic acids research · 2024Article
- Highly sensitive mapping ofbioRxiv : the preprint server for biology · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Genome-wide mapping of fluoroquinolone-stabilized DNA gyrase cleavage sites displays drug specific effects that correlate with bacterial persistence.Nucleic acids research · 2023Article
- DNA supercoiling in bacteria: state of play and challenges from a viewpoint of physics based modeling.Frontiers in microbiology · 2023Review
- Single-nucleotide resolution detection of Topo IV cleavage activity in theFrontiers in microbiology · 2023Article
- MukBEF-dependent chromosomal organization in widenedFrontiers in microbiology · 2023Article
- Host nucleases generate prespacers for primed adaptation in theScience advances · 2022Article
- Intersubunit and intrasubunit interactions driving the MukBEF ATPase.The Journal of biological chemistry · 2022Article
- Psoralen mapping reveals a bacterial genome supercoiling landscape dominated by transcription.Nucleic acids research · 2022Article
- Extended sister-chromosome catenation leads to massive reorganization of the E. coli genome.Nucleic acids research · 2022Article
- The MukB-topoisomerase IV interaction mutually suppresses their catalytic activities.Nucleic acids research · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catenation links between sister chromatids are formed progressively during DNA replication and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in E. coli using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the E. coli chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chromatin and gene expression context influence site selection. Moreover strong DNA-binding and catalytic activities are found at the chromosome dimer resolution site, dif, located opposite the origin of replication. We reveal a physical and functional interaction between Topo IV and the XerCD recombinases acting at the dif site. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.