ArticlePloS one2016
RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Discovery and experimentally mice model validation of CFI, a natural killer T cell-related gene, as a key biomarker in osteoarthritis.Clinical and experimental immunology · 2025Article
- A Microarray Study of Articular Cartilage in Relation to Obesity and Severity of Knee Osteoarthritis.Cartilage · 2020Article
- Gene Expression in Meniscal Tears at the Time of Arthroscopic Partial Meniscectomy Predicts the Progression of Osteoarthritis Within 6 Years of Surgery.Orthopaedic journal of sports medicine · 2020Article
- Duration of symptoms prior to partial meniscectomy is not associated with the expression of osteoarthritis genes in the injured meniscus.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Distinct degenerative phenotype of articular cartilage from knees with meniscus tear compared to knees with osteoarthritis.Osteoarthritis and cartilage · 2019Article
- Microarray analysis of the molecular mechanisms associated with age and body mass index in human meniscal injury.Molecular medicine reports · 2019Article
- Article
- Intra-articular drug delivery systems for joint diseases.Current opinion in pharmacology · 2018Review
- Transcriptome comparison of meniscus from patients with and without osteoarthritis.Osteoarthritis and cartilage · 2018Article
- Advantages of RNA-seq compared to RNA microarrays for transcriptome profiling of anterior cruciate ligament tears.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Bone-cartilage crosstalk: a conversation for understanding osteoarthritis.Bone research · 2016Review
- Gene expression in human meniscal tears has limited association with early degenerative changes in knee articular cartilage.Connective tissue researchArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectives(i) To provide baseline knowledge of gene expression in macroscopically normal articular cartilage, (ii) to test the hypothesis that age, body-mass-index (BMI), and sex are associated with cartilage RNA transcriptome, and (iii) to predict individuals at potential risk for developing "pre-osteoarthritis" (OA) based on screening of genetic risk-alleles associated with OA and gene transcripts differentially expressed between normal and OA cartilage.
designHealthy-appearing cartilage was obtained from the medial femoral notch of 12 knees with a meniscus tear undergoing arthroscopic partial meniscectomy. Cartilage had no radiographic, magnetic-resonance-imaging or arthroscopic evidence for degeneration. RNA was subjected to Affymetrix microarrays followed by validation of selected transcripts by microfluidic digital polymerase-chain-reaction. The underlying biological processes were explored computationally. Transcriptome-wide gene expression was probed for association with known OA genetic risk-alleles assembled from published literature and for comparison with gene transcripts differentially expressed between healthy and OA cartilage from other studies.
resultsWe generated a list of 27,641 gene transcripts in healthy cartilage. Several gene transcripts representing numerous biological processes were correlated with age and BMI and differentially expressed by sex. Based on disease-specific Ingenuity Pathways Analysis, gene transcripts associated with aging were enriched for bone/cartilage disease while the gene expression profile associated with BMI was enriched for growth-plate calcification and OA. When segregated by genetic risk-alleles, two clusters of study patients emerged, one cluster containing transcripts predicted by risk studies. When segregated by OA-associated gene transcripts, three clusters of study patients emerged, one of which is remarkably similar to gene expression pattern in OA.
conclusionsOur study provides a list of gene transcripts in healthy-appearing cartilage. Preliminary analysis into groupings based on OA risk-alleles and OA-associated gene transcripts reveals a subset of patients expressing OA transcripts. Prospective studies in larger cohorts are needed to assess whether these patterns are predictive for OA.
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