ArticlePloS one2016
Maternal Smoking during Pregnancy and DNA-Methylation in Children at Age 5.5 Years: Epigenome-Wide-Analysis in the European Childhood Obesity Project (CHOP)-Study.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 97 citations in OpenAlex.
- Assessment of aberrant DNA methylation two years after paediatric critical illness: a pre-planned secondary analysis of the international PEPaNIC trial.Epigenetics · 2023Trial
- Trial
- Association of prenatal exposure to opioids, cannabis, and polysubstance use with cord blood DNA methylation patterns in a multiancestry cohort.Epigenetics · 2026Article
- Tobacco Smoke Exposure From Prenatal To Adolescent Periods Drives IBD Pathogenesis: Dynamic DNA Methylation Signatures Across Lifespan Stages.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Differential Methylation by Early Life Adversity in the Future of Families Child Wellbeing Study.bioRxiv : the preprint server for biology · 2026Article
- Harnessing machine learning in contemporary tobacco research.Toxicology reports · 2025Review
- Association of exposure to second-hand smoke during childhood with blood DNA methylation.Environment international · 2025Article
- Maternal smoking during pregnancy is associated with DNA methylation in early adolescence: A sibling comparison design.Developmental psychology · 2024Article
- Consistent cord blood DNA methylation signatures of gestational age between South Asian and white European cohorts.Clinical epigenetics · 2024Article
- Blood and urine multi-omics analysis of the impact of e-vaping, smoking, and cessation: from exposome to molecular responses.Scientific reports · 2024Article
- The links between parental smoking and childhood obesity: data of the longitudinal study of Australian children.BMC public health · 2024Article
- Prenatal exposure to maternal smoking and adult lung cancer risk: a nested case-control study using peripheral blood leukocyte DNA methylation prediction of exposure.Environmental epigenetics · 2024Article
- NeonatalToxics · 2023Article
- Polymorphisms in Glutathione S-Transferase (Genes · 2023Article
- Phenome-wide analyses identify an association between the parent-of-origin effects dependent methylome and the rate of aging in humans.Genome biology · 2023Article
- Sex-specific DNA methylation and associations withEpigenetics · 2022Article
- DNA methylation in blood cells is associated with cortisol levels in offspring of mothers who had prenatal post-traumatic stress disorder.Stress and health : journal of the International Society for the Investigation of Stress · 2022Article
- Progresses in epigenetic studies of asthma from the perspective of high-throughput analysis technologies: a narrative review.Annals of translational medicine · 2022Review
- Validation of differential DNA methylation in newborns exposed to tobacco smoke during gestation using bisulfite pyrosequencing.microPublication biology · 2022Article
- DNA methylome perturbations: an epigenetic basis for the emergingly heritable neurodevelopmental abnormalities associated with maternal smoking and maternal nicotine exposure†.Biology of reproduction · 2021Review
Corrections and comments
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Authors and funding
16 authors at 7 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mounting evidence links prenatal exposure to maternal tobacco smoking with disruption of DNA methylation (DNAm) profile in the blood of infants. However, data on the postnatal stability of such DNAm signatures in childhood, as assessed by Epigenome Wide Association Studies (EWAS), are scarce. Objectives of this study were to investigate DNAm signatures associated with in utero tobacco smoke exposure beyond the 12th week of gestation in whole blood of children at age 5.5 years, to replicate previous findings in young European and American children and to assess their biological role by exploring databases and enrichment analysis. DNA methylation was measured in blood of 366 children of the multicentre European Childhood Obesity Project Study using the Illumina Infinium HM450 Beadchip (HM450K). An EWAS was conducted using linear regression of methylation values at each CpG site against in utero smoke exposure, adjusted for study characteristics, biological and technical effects. Methylation levels at five HM450K probes in MYO1G (cg12803068, cg22132788, cg19089201), CNTNAP2 (cg25949550), and FRMD4A (cg11813497) showed differential methylation that reached epigenome-wide significance according to the false-discovery-rate (FDR) criteria (q-value<0.05). Whereas cg25949550 showed decreased methylation (-2% DNAm ß-value), increased methylation was observed for the other probes (9%: cg12803068; 5%: cg22132788; 4%: cg19089201 and 4%: cg11813497) in exposed relative to non-exposed subjects. This study thus replicates previous findings in children ages 3 to 5, 7 and 17 and confirms the postnatal stability of MYO1G, CNTNAP2 and FRMD4A differential methylation. The role of this differential methylation in mediating childhood phenotypes, previously associated with maternal smoking, requires further investigation.
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