ReviewApplied microbiology and biotechnology2016
Tunable recombinant protein expression in E. coli: enabler for continuous processing?
Review in Applied microbiology and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- A single vector system for tunable and homogeneous dual gene expression in Escherichia coli.Scientific reports · 2025Article
- Identification and analysis of the key genes for Escherichia coli heterologous protein expression by transcriptomic profiling.Molecular biology reports · 2024Article
- Article
- Tp0684, Tp0750, and Tp0792 Recombinant Proteins as Antigens for the Serodiagnosis of Syphilis.Indian journal of microbiology · 2022Article
- PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed inComputational and structural biotechnology journal · 2022Article
- Synthetic protein quality control to enhance full-length translation in bacteria.Nature chemical biology · 2021Article
- Green Process: Improved Semi-Continuous Fermentation ofFrontiers in bioengineering and biotechnology · 2021Article
- Fluoride-Controlled Riboswitch-Based Dampening of Gene Expression for Cloning Potent Promoters.Frontiers in genetics · 2021Article
- Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme MaturationFrontiers in bioengineering and biotechnology · 2020Article
- Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing WithFrontiers in bioengineering and biotechnology · 2020Article
- Developing a codon optimization method for improved expression of recombinant proteins in actinobacteria.Scientific reports · 2019Article
- The Rocky Road From Fed-Batch to Continuous Processing WithFrontiers in bioengineering and biotechnology · 2019Review
- Boosting Recombinant Inclusion Body Production-From Classical Fed-Batch Approach to Continuous Cultivation.Frontiers in bioengineering and biotechnology · 2019Article
- Tuning recombinant protein expression to match secretion capacity.Microbial cell factories · 2018Article
- Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms.Microorganisms · 2018Review
- Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli.Applied microbiology and biotechnology · 2018Article
- Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3).Bioengineering (Basel, Switzerland) · 2017Article
- Optimizing the expression of a Heterologous chitinase: A study of different promoters.Bioengineered · 2017Article
- A novel toolbox for E. coli lysis monitoring.Analytical and bioanalytical chemistry · 2017Article
- Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints.Applied microbiology and biotechnology · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuning of transcription is a powerful process technological tool for efficient recombinant protein production in Escherichia coli. Many challenges such as product toxicity, formation of inclusion bodies, cell death, and metabolic burden are associated with non-suitable (too high or too low) levels of recombinant protein expression. Tunable expression systems allow adjusting the recombinant protein expression using process technological means. This enables to exploit the cell's metabolic capacities to a maximum. Within this article, we review genetic and process technological aspects of tunable expression systems in E. coli, providing a roadmap for the industrial exploitation of the reviewed technologies. We attempt to differentiate the term "expression tuning" from its inflationary use by providing a concise definition and highlight interesting fields of application for this versatile new technology. Dependent on the type of inducer (metabolizable or non-metabolizable), different process strategies are required in order to achieve tuning. To fully profit from the benefits of tunable systems, an independent control of growth rate and expression rate is indispensable. Being able to tackle problems such as long-term culture stability and constant product quality expression tuning is a promising enabler for continuous processing in biopharmaceutical production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.