ArticleMolecular biology of the cell2016
Heterogeneous architecture of vertebrate kinetochores revealed by three-dimensional superresolution fluorescence microscopy.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- CCAN forms the spherical shell-like structure in interphase kinetochores.Science advances · 2026Article
- The CENP-A chaperone complex spatially organizes centromeres.Science advances · 2026Article
- The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Visualization of the three-dimensional structure of the human centromere in mitotic chromosomes by superresolution microscopy.Molecular biology of the cell · 2023Article
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Dynamics of kinetochore structure and its regulations during mitotic progression.Cellular and molecular life sciences : CMLS · 2020Review
- CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes.The Journal of cell biology · 2020Article
- CENP-A nucleosome clusters form rosette-like structures around HJURP during G1.Nature communications · 2019Article
- The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.The Journal of cell biology · 2019Article
- Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis.Nature cell biology · 2018Article
- Non-random Mis-segregation of Human Chromosomes.Cell reports · 2018Article
- Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.Scientific reports · 2018Article
- Molecular mechanism of dynein recruitment to kinetochores by the Rod-Zw10-Zwilch complex and Spindly.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The kinetochore is often depicted as having a disk-like architecture in which the outer layer of proteins, which engage microtubules and control checkpoint signaling, are built on a static inner layer directly linked to CENP-A chromatin. Here, applying three-dimensional (3D) structural illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to Xenopus egg extracts and tissue culture cells, we report various distribution patterns of inner and outer kinetochore proteins. In egg extracts, a configuration in which outer kinetochore proteins surround the periphery of CENP-A chromatin is common, forming an ∼200-nm ring-like organization that may engage a bundle of microtubule ends. Similar rings are observed in Xenopus tissue culture cells at a lower frequency but are enriched in conditions in which the spindle is disorganized. Although rings are rare in human cells, the distribution of both inner and outer kinetochore proteins elongates in the absence of microtubule attachment in a manner dependent on Aurora B. We propose a model in which the 3D organization of both the outer and inner kinetochore regions respond to the progression from lateral to end-on microtubule attachments by coalescing into a tight disk from less uniform distributions early in prometaphase.
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