Evidence map›Paper›PMID 27168102›Full record

ArticleNature communications2016

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.

Pranjul Shah, Joëlle V Fritz, Enrico Glaab, Mahesh S Desai, Kacy Greenhalgh, Audrey Frachet, Magdalena Niegowska, Matthew Estes, Christian Jäger, Carole Seguin-Devaux and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 316 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
316citing papers in PubMed, 1 pooled it
25.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

316 citing papers in PubMed, 1 synthesis or guideline pooled it, 634 citations in OpenAlex.

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  7. Establishment of anmSystems · 2026
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256 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Pranjul ShahLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Joëlle V FritzLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Enrico GlaabLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Mahesh S DesaiLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Kacy GreenhalghLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Audrey FrachetLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Magdalena NiegowskaLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Matthew EstesCenter for Applied Nanobioscience and Medicine, University of Arizona, 145S 79th Street, Suite 16, Chandler, Arizona 85226, USA.
Christian JägerLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
Carole Seguin-DevauxDepartment of Infection and Immunity, Luxembourg Institute of Health, 29 rue Henri Koch, Esch-sur-Alzette L-4354, Luxembourg.
Frederic ZenhausernCenter for Applied Nanobioscience and Medicine, University of Arizona, 145S 79th Street, Suite 16, Chandler, Arizona 85226, USA.
Paul WilmesLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 6 avenue du Swing, Belvaux L-4367, Luxembourg.
University of Luxembourg · LUUniversity of Arizona · USLuxembourg Institute of Health · LU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

Indexed as

Gastrointestinal MicrobiomeModels, BiologicalAerobiosisAnaerobiosisBacteriaCaco-2 CellsCoculture TechniquesGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMetabolomicsMicrofluidicsMicroRNAsReproducibility of ResultsMicroRNAs

Identifiers

PMID27168102
PMCPMC4865890
OpenAlexW2359747920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.