ArticleBiotechnology letters2016
Three-dimensional perfused tumour spheroid model for anti-cancer drug screening.
Article in Biotechnology letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Unlocking the Potential of Spheroids in Personalized Medicine: A Systematic Review of Seeding Methodologies.International journal of molecular sciences · 2025Pooled it
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Immune-driven stromal inflammation in pancreatic cancer within a microfluidic platform.Biofabrication · 2026Article
- An oxygen gradient chamber with simple geometry for generating oxygen gradients in hydrogel-based cell cultures within a well-plate-compatible format.Frontiers in bioengineering and biotechnology · 2026Article
- Article
- Three-dimensional heterotypic colorectal cancer spheroid models for evaluation of drug response.Frontiers in oncology · 2023Review
- Organic electronic transmembrane device for hosting and monitoring 3D cell cultures.Science advances · 2022Article
- Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction.Materials today. Bio · 2022Article
- PE38-based gene therapy of HER2-positive breast cancer stem cells via VHH-redirected polyamidoamine dendrimers.Scientific reports · 2021Article
- 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants.Cells · 2021Article
- Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research.Pharmaceutics · 2020Review
- Controllable Assembly of Upconversion Nanoparticles Enhanced Tumor Cell Penetration and Killing Efficiency.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Article
- Characterization of Triple-Negative Breast Cancer MDA-MB-231 Cell Spheroid Model.OncoTargets and therapy · 2020Article
- Advances in Modeling the Immune Microenvironment of Colorectal Cancer.Frontiers in immunology · 2020Review
- Probing tumor microtissue formation and epithelial-mesenchymal transition on a well-mesh microchip.Biomicrofluidics · 2019Article
- pH-sensitive PEGylation of RIPL peptide-conjugated nanostructured lipid carriers: design and in vitro evaluation.International journal of nanomedicine · 2018Article
- Methods to Evaluate Cell Growth, Viability, and Response to Treatment in a Tissue Engineered Breast Cancer Model.Scientific reports · 2017Article
- Perfused Three-dimensional Organotypic Culture of Human Cancer Cells for Therapeutic Evaluation.Scientific reports · 2017Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo build an in vitro-perfused, three-dimensional (3D) spheroid model based on the TissueFlex system for anti-cancer drug efficacy testing in order to mimic avascular micro-tissues with inherent O2, nutrient and metabolite gradients, and to provide a more accurate prediction of drug toxicity and efficacy than traditional in vitro tumour models in conventional static culture well plates.
resultsThe perfused cancer spheroid model showed higher cell viability and increased diameter of spheroids over a relatively long culture period (17 days). Three anti-cancer drugs with different cytotoxic mechanisms were tested. In perfusion, lower cytotoxicity was observed for traditional cytotoxic drug 5-fluorouracil and microtubule-interfering, paclitaxel, showed greater interruption of spheroid integrity. For the hypoxic-dependent drug, tirapazamine, there was no significant difference observed between static and perfusion cultures.
conclusionThe perfusion culture provides a better homeostasis for cancer cell growth in a more controllable working platform for long-term drug testing.
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