ArticleJournal of visualized experiments : JoVE2016
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- [Current concepts and new developments of 3D printing in trauma surgery].Der Unfallchirurg · 2019Pooled it
- Handheld Bioprinters in Skin Regeneration: Current Landscape, Clinical Promise, and the Road Ahead.Biomedicines · 2026Review
- Transformation of the Biological Paradigm in Bone Regeneration: An Integrative Review.Journal of developmental biology · 2026Review
- Spatial patterning strategies for liver tissue engineering: Biofabrication technologies and applications.Advanced drug delivery reviews · 2026Review
- Application of Induced Pluripotent Stem Cells (iPSCs) in Hereditary and Viral Diseases of the Liver: Modeling and Treatment.International journal of molecular sciences · 2025Review
- Review
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- 3D bioprinting of liver models: A systematic scoping review of methods, bioinks, and reporting quality.Materials today. Bio · 2024Article
- Reproducible generation of human liver organoids (HLOs) on a pillar plate platformLab on a chip · 2024Article
- Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications.Tissue engineering and regenerative medicine · 2024Review
- Application of 3D Bioprinting in Liver Diseases.Micromachines · 2023Review
- Recent Developments in 3D Bio-Printing and Its Biomedical Applications.Pharmaceutics · 2023Review
- Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing.Bioactive materials · 2022Review
- Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications.Journal of visualized experiments : JoVE · 2022Article
- Exploiting maleimide-functionalized hyaluronan hydrogels to test cellular responses to physical and biochemical stimuli.Biomedical materials (Bristol, England) · 2022Article
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction.Chinese medicine · 2021Article
- Bioengineering Approaches for the Advanced Organoid Research.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- Developments and Opportunities for 3D Bioprinted Organoids.International journal of bioprinting · 2021Review
- Recapitulating TumorigenesisFrontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Bioprinting has emerged as a versatile biofabrication approach for creating tissue engineered organ constructs. These constructs have potential use as organ replacements for implantation in patients, and also, when created on a smaller size scale as model "organoids" that can be used in in vitro systems for drug and toxicology screening. Despite development of a wide variety of bioprinting devices, application of bioprinting technology can be limited by the availability of materials that both expedite bioprinting procedures and support cell viability and function by providing tissue-specific cues. Here we describe a versatile hyaluronic acid (HA) and gelatin-based hydrogel system comprised of a multi-crosslinker, 2-stage crosslinking protocol, which can provide tissue specific biochemical signals and mimic the mechanical properties of in vivo tissues. Biochemical factors are provided by incorporating tissue-derived extracellular matrix materials, which include potent growth factors. Tissue mechanical properties are controlled combinations of PEG-based crosslinkers with varying molecular weights, geometries (linear or multi-arm), and functional groups to yield extrudable bioinks and final construct shear stiffness values over a wide range (100 Pa to 20 kPa). Using these parameters, hydrogel bioinks were used to bioprint primary liver spheroids in a liver-specific bioink to create in vitro liver constructs with high cell viability and measurable functional albumin and urea output. This methodology provides a general framework that can be adapted for future customization of hydrogels for biofabrication of a wide range of tissue construct types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.