Evidence map›Paper›PMID 27166148›Full record

ArticleNeurochemistry international2016

Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytes.

Deepa Ajit, Agnes Simonyi, Runting Li, Zihong Chen, Mark Hannink, Kevin L Fritsche, Valeri V Mossine, Robert E Smith, Thomas K Dobbs, Rensheng Luo and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemistry international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 2 pooled it
7.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 47 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  4. Review
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  6. Article
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  8. Review
  9. Article
  10. Article
  11. Review
  12. The benefits of ashwagandha (Frontiers in nutrition · 2024
    Review
  13. What We Know aboutNutrients · 2023
    Article
  14. Açaí (Nutrients · 2023
    Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Deepa AjitBiochemistry Department, University of Missouri, Columbia, MO, USA.
Agnes SimonyiBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Runting LiBiochemistry Department, University of Missouri, Columbia, MO, USA.
Zihong ChenBiochemistry Department, University of Missouri, Columbia, MO, USA.
Mark HanninkBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Kevin L FritscheDepartment of Animal Sciences, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Valeri V MossineBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Robert E SmithU.S. FDA, Lenexa, KS, USA.
Thomas K DobbsU.S. FDA, Lenexa, KS, USA.
Rensheng LuoDepartment of Chemistry and Biochemistry, University of Missouri, St. Louis, MO, USA.
William R FolkBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Zezong GuDepartment of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Dennis B LubahnBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
Gary A WeismanBiochemistry Department, University of Missouri, Columbia, MO, USA.
Grace Y SunBiochemistry Department, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA. Electronic address: sung@missouri.edu.
University of Missouri · USUnited States Food and Drug Administration · USUniversity of Missouri–St. Louis · US

Funding

The Interactions Core/Mark McintoshP50AT006273 · NCCIH · UNIVERSITY OF MISSOURI-COLUMBIA · PI LUBAHN, DENNIS B · 2010 to 2014
$7.4M
NCCIH NIH HHS P50 AT006273
6 · The paper itself

Abstract

The increase in oxidative stress and inflammatory responses associated with neurodegenerative diseases has drawn considerable attention towards understanding the transcriptional signaling pathways involving NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and Nrf2 (Nuclear Factor Erythroid 2-like 2). Our recent studies with immortalized murine microglial cells (BV-2) demonstrated effects of botanical polyphenols to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) and enhance Nrf2-mediated antioxidant responses (Sun et al., 2015). In this study, an immortalized rat astrocyte (DI TNC1) cell line expressing a luciferase reporter driven by the NF-κB or the Nrf2/Antioxidant Response Element (ARE) promoter was used to assess regulation of these two pathways by phytochemicals such as quercetin, rutin, cyanidin, cyanidin-3-O-glucoside, as well as botanical extracts from Withania somnifera (Ashwagandha), Sutherlandia frutescens (Sutherlandia) and Euterpe oleracea (Açaí). Quercetin effectively inhibited LPS-induced NF-κB reporter activity and stimulated Nrf2/ARE reporter activity in DI TNC1 astrocytes. Cyanidin and the glycosides showed similar effects but only at much higher concentrations. All three botanical extracts effectively inhibited LPS-induced NF-κB reporter activity. These extracts were capable of enhancing ARE activity by themselves and further enhanced ARE activity in the presence of LPS. Quercetin and botanical extracts induced Nrf2 and HO-1 protein expression. Interestingly, Ashwagandha extract was more active in inducing Nrf2 and HO-1 expression in DI TNC1 astrocytes as compared to Sutherlandia and Açaí extracts. In summary, this study demonstrated NF-kB and Nrf2/ARE promoter activities in DI TNC1 astrocytes, and further showed differences in ability for specific botanical polyphenols and extracts to down-regulate LPS-induced NF-kB and up-regulate the NRF2/ARE activities in these cells.

Indexed as

AnimalsAntioxidant Response ElementsAstrocytesCell Line, TransformedCells, CulturedNF-E2-Related Factor 2NF-kappa BPhytochemicalsPlant ExtractsRatsNF-E2-Related Factor 2NF-kappa BPhytochemicalsPlant ExtractsAçaíARE reporterAshwagandhaNF-κB reporterQuercetinSutherlandia

Identifiers

PMID27166148
PMCPMC4900906
OpenAlexW2347120927

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.